Themed collection 150th Anniversary Collection: Separation Science

Supervised machine learning is used to create a predictive model of differential ion mobility.

First proof of concept of the coupling between normal-phase liquid chromatography and electron ionization mass spectrometry using a Liquid Electron Ionization (LEI) interface.

Samples collected from brain of living subjects are segmented to nanoliter droplets and infused to the mass spectrometer. Temporal resolution for in vivo concentration dynamics of key neurotransmitters of 10 s is achieved. Created with Biorender.com.

GC-APPI-FTICR MS is particularly efficient for the characterization of complex organic mixtures such as plastic pyrolysis oil. The use of dopants allows control of ionization efficiency and selectivity to target specific families of molecules.

This work describes the development of a new approach to measure drug levels and lipid fingerprints in single living cells.

A total of 324 compounds were systematically characterized by feature-based molecular networking. This work provides an efficient strategy for the rapid discovery and characterization of unknown compounds in natural products.

The human body secretes a complex blend of volatile organic compounds (VOCs) via the skin, breath and bodily fluids. In this study, we have developed a headspace collection chamber for whole body volatilome profiling.

The HPLC-SERS hyphenated system exhibited the complementary capability of on-line separation and continuous structural identification. It was extended to the application in identifying the illegally added hypoglycemic drugs in the practical dietary supplements.

Reverse phase solid phase extraction matrices were tested using standard analytes then exposed to space flight stresses to ensure functionality when deployed for liquid sample analysis on remote bodies.

This work presents novel methods of intact protein analysis using surfactant coated neutral dimethyl polysiloxane capillaries for rapid and targeted selection of appropriate background electrolytes via consideration of protein isoelectric points.

Multiplexed immunoaffinity monoliths formed in 3D printed microfluidic devices facilitate extraction and elution to purify and preconcentrate preterm risk biomarkers in blood serum.

Schematic presentation of the in situ growth of COF-TzDa onto the inner wall of capillary for open-tubular capillary electrochromatography.

The use of both 50 cm and 200 cm micro pillar array column (μPAC) for the analysis of permethylated glycan is demonstrated and assessed.

C-CP fiber spin-down tips are employed on a table-top centrifuge to isolate extracellular vesicles from diverse biofluids in less than 15 min. The EVs are isolated in high yields and purity, with the recoveries verified across multiple methods.

The Distribution Function Ratio uses a single, macroscopic chromatogram to assess surface dynamics usually quantified using complex microscopy experiments.

We developed a novel fast gas chromatography (fastGC) instrument with integrated sampling of volatile organic compounds (VOCs) and detection by single-photon ionisation (SPI) time-of-flight mass spectrometry (TOFMS).

A new ambient ionization method, 3D-printed cone spray ionization mass spectrometry (3D-PCSI-MS), is implemented for rapid profiling of chemical warfare agent (CWA) simulants in unprepared soil samples.

Sampling via liquid extraction pen and electrospray ionization allows for rapid, sensitive, and semi-quantitative mass spectrometric analyses of daily goods.

A modified derivatisation-based method allowed for the measurement of free and conjugated estrogens using LC-MS and its application for monitoring compounds of interest in natural water was validated.

A fully automated novel workflow for lipidomics based on flow injection, followed by reversed phase liquid chromatography–high-resolution mass spectrometry in combination with LILY as internal standard, enabling accurate quantification via RP-LC.