Themed collection Analytical Methods HOT Articles 2025

Hydrogel-assisted chemiluminescence and electrochemiluminescence systems for bioanalytical applications
The novelty of this review lies in the comprehensive exploration of hydrogel-assisted chemiluminescence and electrochemiluminescence systems for bioanalytical applications.
Anal. Methods, 2025,17, 5546-5562
https://doi.org/10.1039/D5AY00489F
Research progress on regulation strategies for surface-enhanced Raman spectroscopy
This review summarizes the latest regulation strategies based on the physical and chemical enhancement.
Anal. Methods, 2025,17, 4482-4495
https://doi.org/10.1039/D5AY00555H
Carbon-Based Nanozymes: Catalytic Mechanisms, Performance Tuning, and Environmental-Biomedical Applications
Anal. Methods, 2025, Accepted Manuscript
https://doi.org/10.1039/D5AY00997A
Advancing food safety and quality assessment: a comprehensive review of non-destructive analytical technologies
A comprehensive summary of the principles and applications of non-destructive analytical technologies.
Anal. Methods, 2025,17, 4697-4717
https://doi.org/10.1039/D5AY00647C
Empowering protein single-molecule sequencing: nanopore technology toward sensing gene sequences
The investigation of proteins at the single-molecule level is urgent to reveal the relationship between their structure and function.
Anal. Methods, 2025,17, 3902-3924
https://doi.org/10.1039/D5AY00572H
Target-induced nanoparticle assemblies: a comprehensive review of strategies for nucleic acid functionalization, biosensing, and drug delivery applications
Various applications of target-induced nanoparticle assemblies.
Anal. Methods, 2025,17, 3876-3901
https://doi.org/10.1039/D5AY00413F
Rapid antibiotic biosensors based on multiple molecular recognition elements
The molecular recognition elements utilized for antibiotic detection are presented, along with the interactions between them. The inner circle represents the five types of intermolecular forces, while the outer circle embodies the seven molecular recognition elements and direct recognition.
Anal. Methods, 2025,17, 2496-2514
https://doi.org/10.1039/D4AY02212B

A portable and ecological paper-based device for glucose monitoring in peripheral blood mononuclear cell lysates
The increasing need for point-of-care (POC) testing has prompted a rise in the popularity of affordable biosensors that are eco-friendly, especially paper-based electrochemical sensors.
Anal. Methods, 2025,17, 2529-2535
https://doi.org/10.1039/D5AY00057B
A microfluidic DNA sensor array for real-time screening of early-stage lung cancer by simultaneous detection of multiple miRNAs
A microfluidic capacitive DNA sensor array is developed for simultaneous detection of four targeted miRNAs in serum for early-stage lung cancer screening, with a low detection limit and real-time response.
Anal. Methods, 2025,17, 2376-2379
https://doi.org/10.1039/D5AY00109A

Sustainability from the start: biochar-based conductive inks enable the streamlined fabrication of green electroanalytical devices
A green conductive ink containing biochar from peanut shells used to print disposable electrodes.
Anal. Methods, 2025, Advance Article
https://doi.org/10.1039/D5AY00740B
Harnessing zinc(II) chelation for fluorescence determination of besifloxacin in ophthalmic and biological matrices
A sensitive spectrofluorimetric method boosts besifloxacin fluorescence by forming a 2 : 1 BFN–Zn2+ complex at pH 6.0, enabling its accurate determination in pure form, eye drops, and spiked aqueous humor.
Anal. Methods, 2025,17, 5860-5867
https://doi.org/10.1039/D5AY00894H
Analysis of pyrolysis component variations in three azo compounds using headspace gas chromatography-mass spectrometry (HS-GC-MS)
The pyrolysis products of three azo compounds were examined using the HS-GC-MS method, and the classification and identification of azo compounds were achieved through PAC, OPLS-DA, and Fisher discriminant analysis models.
Anal. Methods, 2025,17, 5849-5859
https://doi.org/10.1039/D5AY00739A
Sustainable Headspace-In-needle Microextraction Analysis of Phthalates Using Biomass-derived Carbon Coated Needles
Anal. Methods, 2025, Accepted Manuscript
https://doi.org/10.1039/D5AY00221D
An environmentally relevant solid-state sensor for the trace detection of triclopyr herbicide
A novel solid-contact potentiometric sensor was developed for the selective and trace-level detection of triclopyr (Tcpy), an environmentally persistent pyridine-based herbicide.
Anal. Methods, 2025, Advance Article
https://doi.org/10.1039/D5AY00916B
Upconversion fluorescence immunochromatographic assay for highly sensitive and rapid detection of ovarian function indicators: follicle-stimulating hormone and anti-Müllerian hormone
A novel, highly sensitive detection method enables both rapid quantitative detection and visual qualitative analysis of FSH/AMH via upconversion fluorescence.
Anal. Methods, 2025, Advance Article
https://doi.org/10.1039/D5AY00500K
Characterization of non-volatile compounds, volatile compounds, and inorganic elements in different growth stages of Scutellariae Radix
Scutellariae Radix, which is primarily used in clinical settings in two forms namely Ziqin and Kuqin, exerts different effects due to the differences in their content of volatile, non-volatile, and inorganic components.
Anal. Methods, 2025, Advance Article
https://doi.org/10.1039/D5AY00762C
Colorimetric detection of L-Dopa via anti-etching of Au nanorods catalyzed by an MIL-88A (Fe)-TMB system
A colorimetric sensor based on the inhibition of gold nanorod etching by L-Dopa is developed, enabling simple and visual detection of L-Dopa via MOF-catalyzed TMB oxidation suppression.
Anal. Methods, 2025, Advance Article
https://doi.org/10.1039/D5AY00805K
A dual-mode detection strategy for alkaline phosphatase activity based on dimeric G-quadruplex
A new fluorescence and colorimetric dual-mode strategy for ALP based on dimeric G-quadruplex was proposed.
Anal. Methods, 2025,17, 5644-5649
https://doi.org/10.1039/D5AY00934K
Application of 2D-liquid chromatography for the separation of a mixture of isomeric and structurally related azatryptophan derivatives
2D-Liquid chromatography was used for the achiral and chiral separation of azatryptophan derivatives. Reverse phase mode for the first-dimension analysis and polar organic mode for the second-dimension analysis were employed.
Anal. Methods, 2025,17, 5650-5661
https://doi.org/10.1039/D5AY00473J
A lysosome targetable and acidic pH-activated fluorescent probe for two-photon imaging of hydrogen sulfide in live cells and animals
The illustration of a lysosome-targeted probe and its in situ response to H2S controlled by the acidic environment in lysosomes.
Anal. Methods, 2025,17, 5372-5382
https://doi.org/10.1039/D5AY00757G
A novel IagPLS baseline correction method for glioma identification using Raman spectroscopy
We propose IagPLS, a novel Raman baseline correction method integrating curvature-driven regularization, SHAP-guided feature protection, and quantum-inspired optimization, significantly enhancing spectral fidelity for glioma detection.
Anal. Methods, 2025,17, 5343-5354
https://doi.org/10.1039/D5AY00792E
A portable optical device for quantitative detection of lithium in blood plasma
This study presents a portable, low-cost optical biosensor for rapid and accurate lithium detection in blood plasma, enabling point-of-care therapeutic drug monitoring for bipolar disorder patients.
Anal. Methods, 2025,17, 5355-5361
https://doi.org/10.1039/D5AY00469A
Dual detection of hypervirulent genes of Klebsiella pneumoniae using a single CRISPR-Cas12a system modulated using entropy-driven circuits
A CRISPR-based dual gene assay was proposed for hypervirulent Klebsiella pneumoniae (hvKp) by integrating two parallel entropy-driven circuits (EDCs) and the trans-cleavage activity of the Cas12a protein towards fluorescent substrate reporters.
Anal. Methods, 2025,17, 5362-5371
https://doi.org/10.1039/D5AY00563A
Conditional regulation of HCR for rapid visualization of endogenous miR-21 in cancer cells
Rapid detection of endogenous miR-21 in living cells was achieved by effectively regulating the HCR reaction conditions, where the presence of Na+&Mg2+ and the sequential addition of H1 and then H2 were necessary.
Anal. Methods, 2025,17, 5170-5175
https://doi.org/10.1039/D5AY00542F
A renewable enzyme-driven dissipative DNA strand displacement strategy for T4 polynucleotide kinase activity assay and inhibitor screening
A fluorescence biosensor based on an enzyme-driven dissipative strand displacement strategy was proposed for T4 polynucleotide kinase activity and inhibitor screening.
Anal. Methods, 2025,17, 4944-4950
https://doi.org/10.1039/D5AY00700C
Detection of bitter limonin in citrus samples using plasmonic gold nanobipyramid-based dual-mode lateral flow immunoassay
A novel plasmonic gold nanobipyramid (AuNBP)-based lateral flow immunoassay (LFIA) has been developed for the colorimetric and photothermal detection of limonin.
Anal. Methods, 2025,17, 4935-4943
https://doi.org/10.1039/D5AY00638D
Controlled fabrication of novel magneto-fluorescent encoded microspheres with host–guest structures for simultaneous detection of thyroxine and thyroid stimulating hormone
Novel host–guest structured magneto-fluorescent encoded microspheres were prepared for constructing a suspension array for simultaneous detection of T4 and TSH.
Anal. Methods, 2025,17, 4734-4744
https://doi.org/10.1039/D5AY00554J
A fluorescence and colorimetric dual-mode LAMP method for detection of Vibrio parahaemolyticus
A dual-mode LAMP method integrating fluorescence detection and visual colorimetric detection was proposed for detection of V. parahaemolyticus. The method has good specificity and can accurately detect 1 pg μL−1 of V. parahaemolyticus DNA.
Anal. Methods, 2025,17, 3925-3932
https://doi.org/10.1039/D5AY00407A
A BODIPY-based fluorescent probe for rapid detection of NO in cells and zebrafish
BDD demonstrates high sensitivity, high selectivity and rapid response performance in the detection of NO. It has been successfully utilized for imaging both exogenous and endogenous NO in cells and zebrafish.
Anal. Methods, 2025,17, 3629-3635
https://doi.org/10.1039/D5AY00460H
A “turn-on” fluorescent sensor for the selective detection of erythromycin in aqueous solution
A novel “turn-on” fluorescent sensor for highly selective and sensitive detection of erythromycin in aqueous solutions.
Anal. Methods, 2025,17, 3636-3643
https://doi.org/10.1039/D4AY02147A
Evaluation of pure shift NMR methods for the analysis of complex metabolite mixtures with a benchtop NMR spectrometer
A comparison of ultra-resolved pure shift NMR techniques, mitigating the resolution problem of compact NMR instruments, showed that the J-resolved double echo is the most performant technique, enabling the profiling of biological complex mixtures.
Anal. Methods, 2025,17, 3171-3182
https://doi.org/10.1039/D5AY00006H
Multichannel microperfusion cell-capture system for screening anticancer active ingredients in traditional Chinese medicine formulas
We established a multichannel microperfusion cell-capture system to screen potential anticancer ingredients in Huangqisan, providing an innovative approach for high-throughput screening of active ingredients in traditional Chinese medicine.
Anal. Methods, 2025,17, 3122-3131
https://doi.org/10.1039/D5AY00205B
Fe–N–C oxidase-mimicking nanozymes for discrimination of antioxidants and detection of Hg2+
Single-atom nanozymes (SAzymes) with high atom utilization efficiency and well-defined coordination structures have been extensively studied.
Anal. Methods, 2025,17, 3145-3151
https://doi.org/10.1039/D5AY00313J
SERS and DFT studies of highly sensitive detection of trace nitro-explosives assisted by alkali ions
This study introduces a simple method for the trace detection of nitro-explosives (CL-20, TNCB, and TNT) using SERS with the assistance of alkali ions.
Anal. Methods, 2025,17, 3152-3163
https://doi.org/10.1039/D5AY00391A
Novel poly-deep eutectic solvent-functionalized magnetic graphene oxide nanomaterials for high-performance solid-phase extraction of trypsin
Three novel polymethacrylic acid-based deep eutectic solvent (DES)-functionalized magnetic graphene oxide composites were designed and successfully synthesized via the radical polymerization method.
Anal. Methods, 2025,17, 3132-3144
https://doi.org/10.1039/D5AY00252D
Novel derivatized cyclodextrin as a stationary phase in gas chromatography for separating chiral and achiral compounds
The development of efficient techniques for chiral separation has become increasingly vital, especially with the increasing demand for optically pure substances in the pharmaceutical industry and other fields.
Anal. Methods, 2025,17, 3164-3170
https://doi.org/10.1039/D5AY00256G
Gaining insights into nutrient and metal element distributions in radix Angelicae sinensis using mass spectrometry
Content distribution of nutrients and metal elements in RAS batches was analyzed for assessing the micronutrient element level and material quality. A new IBR method was introduced to address the statistical features of multiple content responses.
Anal. Methods, 2025,17, 2826-2834
https://doi.org/10.1039/D5AY00106D
An electrochemical DNA biosensor based on denatured vesicle-mediated chain exchange amplification combined with electric field-assistance for nucleic acid detection
An electrochemical DNA biosensor was developed based on SEA, which can detect 1 fg uL−1 genomic DNA within 65 min. The electrode surface was modified with AuNPs to improve sensitivity and the biosensor was accelerated by the electric field.
Anal. Methods, 2025,17, 2657-2664
https://doi.org/10.1039/D5AY00094G
Development of a UPLC-MS/MS assay for determination of PA-PEG8-PA polymers in rat plasma coupled with [M − H]− to enhance sensitivity
Determination of PA-PEG8-PA polymers by UPLC-MS/MS.
Anal. Methods, 2025,17, 2676-2685
https://doi.org/10.1039/D4AY02050B
C-ZIF-8 modified NiO photocathode and enhanced photosensitizer signal amplification for ultra-sensitive photoelectrochemical detection of lead ions
C-ZIF-8 enhanced the NiO photocathode current by reducing the bandgap, promoting charge transfer, and increasing the load of sensitizer, achieving ultra-sensitive detection of Pb2+.
Anal. Methods, 2025,17, 2650-2656
https://doi.org/10.1039/D5AY00136F
Development of stable and sensitive strip biosensors for the rapid detection of organophosphates and aflatoxin B1 in agricultural samples
Silk fibroin biosensor offers exceptional enzyme stability (activity maintained >180 days at 37 °C) and high sensitivity, detecting organophosphates at 6.57 ppb and aflatoxin B1 at 0.274 ppb.
Anal. Methods, 2025,17, 2665-2675
https://doi.org/10.1039/D4AY02229G
Elastic scattering spectrum fused with Raman spectrum for rapid classification of colorectal cancer tissues
A fast and accurate method that is independent of physicians’ experience for the intraoperative examination of colorectal tumors is proposed by fusing elastic scattering spectrum with Raman spectrum and using an AI model called spec-transformer.
Anal. Methods, 2025,17, 2029-2037
https://doi.org/10.1039/D4AY02221A
About this collection
This themed collection features articles published in Analytical Methods marked as HOT by the handling editor or as recommended by referees. Congratulations to all the authors whose articles are featured.