Themed collection Analytical Methods HOT Articles 2025

The novelty of this review lies in the comprehensive exploration of hydrogel-assisted chemiluminescence and electrochemiluminescence systems for bioanalytical applications.

This review summarizes the latest regulation strategies based on the physical and chemical enhancement.

A comprehensive summary of the principles and applications of non-destructive analytical technologies.

The investigation of proteins at the single-molecule level is urgent to reveal the relationship between their structure and function.

Various applications of target-induced nanoparticle assemblies.

The molecular recognition elements utilized for antibiotic detection are presented, along with the interactions between them. The inner circle represents the five types of intermolecular forces, while the outer circle embodies the seven molecular recognition elements and direct recognition.

The increasing need for point-of-care (POC) testing has prompted a rise in the popularity of affordable biosensors that are eco-friendly, especially paper-based electrochemical sensors.

A microfluidic capacitive DNA sensor array is developed for simultaneous detection of four targeted miRNAs in serum for early-stage lung cancer screening, with a low detection limit and real-time response.

A novel solid-contact potentiometric sensor was developed for the selective and trace-level detection of triclopyr (Tcpy), an environmentally persistent pyridine-based herbicide.

A colorimetric sensor based on the inhibition of gold nanorod etching by L-Dopa is developed, enabling simple and visual detection of L-Dopa via MOF-catalyzed TMB oxidation suppression.

Scutellariae Radix, which is primarily used in clinical settings in two forms namely Ziqin and Kuqin, exerts different effects due to the differences in their content of volatile, non-volatile, and inorganic components.

A novel, highly sensitive detection method enables both rapid quantitative detection and visual qualitative analysis of FSH/AMH via upconversion fluorescence.

A green conductive ink containing biochar from peanut shells used to print disposable electrodes.

A sensitive spectrofluorimetric method boosts besifloxacin fluorescence by forming a 2 : 1 BFN–Zn²⁺ complex at pH 6.0, enabling its accurate determination in pure form, eye drops, and spiked aqueous humor.

The pyrolysis products of three azo compounds were examined using the HS-GC-MS method, and the classification and identification of azo compounds were achieved through PAC, OPLS-DA, and Fisher discriminant analysis models.

A new fluorescence and colorimetric dual-mode strategy for ALP based on dimeric G-quadruplex was proposed.

2D-Liquid chromatography was used for the achiral and chiral separation of azatryptophan derivatives. Reverse phase mode for the first-dimension analysis and polar organic mode for the second-dimension analysis were employed.

The illustration of a lysosome-targeted probe and its in situ response to H₂S controlled by the acidic environment in lysosomes.

We propose IagPLS, a novel Raman baseline correction method integrating curvature-driven regularization, SHAP-guided feature protection, and quantum-inspired optimization, significantly enhancing spectral fidelity for glioma detection.

This study presents a portable, low-cost optical biosensor for rapid and accurate lithium detection in blood plasma, enabling point-of-care therapeutic drug monitoring for bipolar disorder patients.

A CRISPR-based dual gene assay was proposed for hypervirulent Klebsiella pneumoniae (hvKp) by integrating two parallel entropy-driven circuits (EDCs) and the trans-cleavage activity of the Cas12a protein towards fluorescent substrate reporters.

Rapid detection of endogenous miR-21 in living cells was achieved by effectively regulating the HCR reaction conditions, where the presence of Na⁺&Mg²⁺ and the sequential addition of H₁ and then H₂ were necessary.

A fluorescence biosensor based on an enzyme-driven dissipative strand displacement strategy was proposed for T4 polynucleotide kinase activity and inhibitor screening.

A novel plasmonic gold nanobipyramid (AuNBP)-based lateral flow immunoassay (LFIA) has been developed for the colorimetric and photothermal detection of limonin.

Novel host–guest structured magneto-fluorescent encoded microspheres were prepared for constructing a suspension array for simultaneous detection of T4 and TSH.

A dual-mode LAMP method integrating fluorescence detection and visual colorimetric detection was proposed for detection of V. parahaemolyticus. The method has good specificity and can accurately detect 1 pg μL⁻¹ of V. parahaemolyticus DNA.

BDD demonstrates high sensitivity, high selectivity and rapid response performance in the detection of NO. It has been successfully utilized for imaging both exogenous and endogenous NO in cells and zebrafish.

A novel “turn-on” fluorescent sensor for highly selective and sensitive detection of erythromycin in aqueous solutions.

A comparison of ultra-resolved pure shift NMR techniques, mitigating the resolution problem of compact NMR instruments, showed that the J-resolved double echo is the most performant technique, enabling the profiling of biological complex mixtures.

We established a multichannel microperfusion cell-capture system to screen potential anticancer ingredients in Huangqisan, providing an innovative approach for high-throughput screening of active ingredients in traditional Chinese medicine.

Single-atom nanozymes (SAzymes) with high atom utilization efficiency and well-defined coordination structures have been extensively studied.

This study introduces a simple method for the trace detection of nitro-explosives (CL-20, TNCB, and TNT) using SERS with the assistance of alkali ions.

Three novel polymethacrylic acid-based deep eutectic solvent (DES)-functionalized magnetic graphene oxide composites were designed and successfully synthesized via the radical polymerization method.

The development of efficient techniques for chiral separation has become increasingly vital, especially with the increasing demand for optically pure substances in the pharmaceutical industry and other fields.

Content distribution of nutrients and metal elements in RAS batches was analyzed for assessing the micronutrient element level and material quality. A new IBR method was introduced to address the statistical features of multiple content responses.

An electrochemical DNA biosensor was developed based on SEA, which can detect 1 fg uL⁻¹ genomic DNA within 65 min. The electrode surface was modified with AuNPs to improve sensitivity and the biosensor was accelerated by the electric field.

Determination of PA-PEG₈-PA polymers by UPLC-MS/MS.

C-ZIF-8 enhanced the NiO photocathode current by reducing the bandgap, promoting charge transfer, and increasing the load of sensitizer, achieving ultra-sensitive detection of Pb²⁺.

Silk fibroin biosensor offers exceptional enzyme stability (activity maintained >180 days at 37 °C) and high sensitivity, detecting organophosphates at 6.57 ppb and aflatoxin B1 at 0.274 ppb.

A fast and accurate method that is independent of physicians’ experience for the intraoperative examination of colorectal tumors is proposed by fusing elastic scattering spectrum with Raman spectrum and using an AI model called spec-transformer.