Enantiomeric fraction evaluation for assessing septic tanks as a pathway for chiral pharmaceuticals entering rivers

Abstract

Septic tanks (STs) are an important pathway for chiral pharmaceuticals entering rivers. Therefore, the enantiospecific compositions of 25 chiral human pharmaceuticals and metabolites were investigated in five community STs over 12 months in Scotland. Large variability in pharmaceutical concentrations and enantiomeric fractions (EFs) were observed in wastewater owing to the small contributing populations. Pharmaceuticals prescribed in enantiopure and racemic forms had the greatest EF variability. For example, citalopram generally had EFs < 0.5 through consumption of the racemate and preferential metabolism of S(+)-citalopram. However, several samples had EFs > 0.7 from comparatively greater use of enantiopure escitalopram. Direct down-the-drain disposal was indicated for citalopram and venlafaxine, where elevated concentrations and pharmaceutical–metabolite-ratios were observed (at least 19-fold). Overall, EF differences between influent and effluent were small, suggesting no enantioselectivity occurred in anaerobic environments of STs. Therefore, EFs in ST effluent were notably different to those from aerobic wastewater treatment works (WWTWs). For instance, naproxen EFs (≥0.990 when both enantiomers detected) were like those of untreated wastewater but outside the range for aerobic WWTWs effluent caused by a lack of inversion from S(+)- to R(−)-naproxen in STs. This suggests naproxen can be used to identify its pathway into the environment, which was strengthened by river water microcosm studies. At the study locations the environmental risk of enantiomers was low due to sufficient dilution of effluents. Nevertheless, greater impact of individual practices towards medicine use and disposal on ST wastewater and receiving water composition demands enantioselective analysis to better appreciate the sources, fate and impact of pharmaceuticals.