Issue 11, 2021

Diffuse reflectance infrared Fourier transform spectroscopy for a qualitative evaluation of plant leaf pigment extraction

Abstract

The extraction and quantification of leaf pigments are easy, fast, and cheap procedures; on the other hand, diffuse reflectance infrared Fourier transform (DRIFT) spectroscopy associated with chemometrics tools could offer new insights into leaf biochemical composition. We aimed to boost the classic leaf pigment quantification, adding leaf biochemical information derived from DRIFT spectroscopy + principal component analysis, using the same leaf pigment extract produced by the classical quantification method. We performed a dose–response experiment using P as the limiting nutrient, and maize (Zea mays L.) as a plant-test. After 45 d of growth, we evaluated the effects of P fertilization in total maize shoot biomass, P shoot accumulation, leaf pigment quantification by UV-Vis, and the evaluation of biochemical variations by DRIFT spectroscopy analysis associated with a chemometric approach in the same leaf extract used for pigment quantification. P fertilization raised biomass accumulation (∼7.4×), P uptake (∼2.3×), and total chlorophyll a and b contents (∼2.1×). DRIFT spectroscopy analysis of extracted pigments revealed an elevated content of proteins and polysaccharides at high P availability. At low P availability, we found a low efficiency of N metabolism suggested by the accumulation of inorganic N forms. DRIFT spectroscopy applied together with the classic leaf pigment extraction and quantification method is a novel and promising tool for plant nutrition studies as a DRIFT spectroscopy metabolic profile protocol.

Graphical abstract: Diffuse reflectance infrared Fourier transform spectroscopy for a qualitative evaluation of plant leaf pigment extraction

Article information

Article type
Communication
Submitted
11 ינו 2021
Accepted
01 אפר 2021
First published
06 מאי 2021
This article is Open Access
Creative Commons BY-NC license

Analyst, 2021,146, 3440-3448

Diffuse reflectance infrared Fourier transform spectroscopy for a qualitative evaluation of plant leaf pigment extraction

M. S. C. Barreto, J. V. D. Reis, T. Muraoka, M. Jemo, L. Vergutz and L. R. F. Alleoni, Analyst, 2021, 146, 3440 DOI: 10.1039/D1AN00059D

This article is licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported Licence. You can use material from this article in other publications, without requesting further permission from the RSC, provided that the correct acknowledgement is given and it is not used for commercial purposes.

To request permission to reproduce material from this article in a commercial publication, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party commercial publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements