Ionic liquid (IL)-functionalized graphene oxide (GO) containing gold nanoparticles (Au NPs) (5.4–5.9 nm) deposited by sputtering combined with cholesterol oxidase appears to be a suitable and efficient biosensor for total cholesterol detection. These biosensors showed good linear range (25–340 μmol L−1) for total cholesterol determination by colorimetric tests. In addition, control experiments in the presence of interferents demonstrated that the hybrid materials provided a selective detection of total cholesterol. The biosensing activities obtained for the hybrid materials indicate that these compounds are potential biosensors for clinical applications.
