Issue 39, 2015
From the journal:

Chemical Communications

Biosynthetic selenoproteins with genetically-encoded photocaged selenocysteines†

Rasa Rakauskaitė,a   Giedrė Urbanavičiūtė,a   Audronė Rukšėnaitė,a   Zita Liutkevičiūtė,‡a   Robertas Juškėnas,ab   Viktoras Masevičiusab  and  Saulius Klimašauskas*a  

* Corresponding authors

a Institute of Biotechnology, Vilnius University, V. Graičiūno 8, Vilnius LT-02241, Lithuania
E-mail: saulius.klimasauskas@bti.vu.lt
Fax: +370 5260116

b Faculty of Chemistry, Vilnius University, Naugarduko 24, Vilnius LT-03225, Lithuania

Abstract

Selenocysteine is a valuable component of both natural selenoproteins and designer biocatalysts; however the availability of such proteins is hampered by technical limitations. Here we report the first general strategy for the production of selenoproteins via genetically-encoded incorporation of a synthetic photocaged selenocysteine residue in yeast cells, and provide examples of light-controlled protein dimerization and targeted covalent labeling in vitro.

Graphical abstract: Biosynthetic selenoproteins with genetically-encoded photocaged selenocysteines

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Article information

DOI
https://doi.org/10.1039/C4CC07910H
Article type
Communication
Submitted
07 okt. 2014
Accepted
05 feb. 2015
First published
05 feb. 2015
This article is Open Access
Creative Commons BY-NC license

Chem. Commun., 2015,51, 8245-8248

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Biosynthetic selenoproteins with genetically-encoded photocaged selenocysteines

R. Rakauskaitė, G. Urbanavičiūtė, A. Rukšėnaitė, Z. Liutkevičiūtė, R. Juškėnas, V. Masevičius and S. Klimašauskas, Chem. Commun., 2015, 51, 8245 DOI: 10.1039/C4CC07910H

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