Issue 12, 2017

A diagnostic test for cocaine and benzoylecgonine in urine and oral fluid using portable mass spectrometry

Abstract

Surface mass spectrometry methods can be difficult to use effectively with low cost, portable mass spectrometers. This is because commercially available portable (single quadrupole) mass spectrometers lack the mass resolution to confidently differentiate between analyte and background signals. Additionally, current surface analysis methods provide no facility for chromatographic separation and therefore are vulnerable to ion suppression. Here we present a new analytical method where analytes are extracted from a sample using a solvent flushed across the surface under high pressure, separated using a chromatography column and then analysed using a portable mass spectrometer. The use of chromatography reduces ion suppression effects and this, used in combination with in-source fragmentation, increases selectivity, thereby allowing high sensitivity to be achieved with a portable and affordable quadrupole mass spectrometer. We demonstrate the efficacy of the method for the quantitative detection of cocaine and benzoylecgonine in urine and oral fluid. The method gives relative standard deviations below 15% (with one exception), and R2 values above 0.998. The limits of detection for these analytes in oral fluid and urine are <30 ng ml−1, which are comparable to the cut-offs currently used in drug testing, making the technique a possible candidate for roadside or clinic-based drug testing.

Graphical abstract: A diagnostic test for cocaine and benzoylecgonine in urine and oral fluid using portable mass spectrometry

Supplementary files

Article information

Article type
Paper
Submitted
14 júl. 2016
Accepted
17 okt. 2016
First published
21 nóv. 2016
This article is Open Access
Creative Commons BY license

Anal. Methods, 2017,9, 1839-1847

A diagnostic test for cocaine and benzoylecgonine in urine and oral fluid using portable mass spectrometry

M. Ismail, M. Baumert, D. Stevenson, J. Watts, R. Webb, C. Costa, F. Robinson and M. Bailey, Anal. Methods, 2017, 9, 1839 DOI: 10.1039/C6AY02006B

This article is licensed under a Creative Commons Attribution 3.0 Unported Licence. You can use material from this article in other publications without requesting further permissions from the RSC, provided that the correct acknowledgement is given.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements