A multifunctional organophosphonic ligand-templated poly(polyoxotungstate) with potential in building a genosensor for thalassemia gene detection

Abstract

A novel P^V–Sb^III heteroatom-inserted poly(polyoxotungstate) (poly(POT)) [H₂N(CH₃)₂]₅Na₁₁H₉[(HEDATMPA)SbW₁₅O₅₀]{[PrW₄O₁₀][B-α-SbW₉O₃₃]₂}·38H₂O (1) was synthesized and is composed of an attractive [(HEDATMPA)SbW₁₅O₅₀]¹⁴⁻ hybrid fragment and one sandwich-like Pr^III-inserted [PrW₄O₁₀(B-α-SbW₉O₃₃)₂]¹¹⁻ entity by using ethylenediamine tetramethylphosphoric acid (H₈EDATMPA) with potential heteroatom sites and functional organic groups. Considering the advantages of poly(POT) clusters in storing and transferring electrons, we fabricated a 1-PEDOT (PEDOT = poly(3,4-ethylenedioxythiophene)) hybrid film, which was used as an electrode-modified material to construct a 1-based genosensor (GS) for detecting the β-thalassemia gene. Research results show that this 1-based GS demonstrates high specificity, outstanding antijamming ability and low detection limit for β-thalassemia gene detection. This work not only provides a novel and feasible preparative strategy to construct poly(POT) clusters, but also promisingly promotes the development of poly(POT)-based GSs.