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A benzimidazole-decorated two-photon fluorescent probe (Lyso-MPCB) based on the p-methoxyphenylacetylene-substituted carbazole was developed for detecting lysosomal pH with a double-channel signal, which can be used to visualize autophagy by real-time imaging the fluctuation of the pH in the lysosomes.

Graphical abstract: Real-time visualization of autophagy by monitoring the fluctuation of lysosomal pH with a ratiometric two-photon fluorescent probe

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