Issue 13, 2023

Separation of flavonoids with significant biological activity from Acacia mearnsii leaves

Abstract

Acacia mearnsii leaves, which are a rich source of flavonoids, were used to separate and purify myricitrin (W3) and myricetin-3-O-glucoside (W1). Further, the antioxidant and hypoglycemic activities of the two purified flavonoids were evaluated. The flavonoids were separated using solvent partition, macroporous adsorbent resin column, and Sephadex column chromatography, and purified using preparative reverse-phase high-performance liquid chromatography (HPLC). The purified flavonoids were characterized using HPLC, mass spectrometry, and nuclear magnetic resonance methods. A high yield (7.3 mg g−1 of crude extract) of W3 was obtained, with a high purity of 98.4%. Furthermore, the purity of W1 was over 95%. W1 and W3 showed strong antioxidant activity and significantly inhibited α-glucosidase. W3 also demonstrated substantial α-amylase inhibitory capacity. This study indicated that A. mearnsii leaves, which are discarded in significant amounts, can be used as a source of myricitrin, thus providing more adequate material for the production of antioxidants and type II diabetes inhibitors. Hence, A. mearnsii leaves have the potential to create great market economic value and environmental benefits.

Graphical abstract: Separation of flavonoids with significant biological activity from Acacia mearnsii leaves

Supplementary files

Article information

Article type
Paper
Submitted
11 1 2023
Accepted
10 3 2023
First published
20 3 2023
This article is Open Access
Creative Commons BY-NC license

RSC Adv., 2023,13, 9119-9127

Separation of flavonoids with significant biological activity from Acacia mearnsii leaves

C. Wu, L. He, Y. Zhang, C. You, X. Li, P. Jiang and F. Wang, RSC Adv., 2023, 13, 9119 DOI: 10.1039/D3RA00209H

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