Issue 40, 2014
From the journal:

Chemical Communications

Investigating the reactivities of a polyketide synthase module through fluorescent click chemistry†

Amanda Jane Hughes,a   Matthew R. Tibby,a   Drew T. Wagner,a   Johnathan N. Brantleya  and  Adrian T. Keatinge-Clay*a  

* Corresponding authors

a Department of Chemistry and Biochemistry, The University of Texas at Austin, 1 University Station A5300, Austin, TX, USA
E-mail: adriankc@utexas.edu
Fax: +1 512 471-8696
Tel: +1 512 471-2977

Abstract

A method for monitoring in vitro polyketide synthesis has been developed whereby nonchromophoric polyketide products are made brightly fluorescent in a simple, rapid, inexpensive, and bioorthogonal manner through CuAAC with a sulforhodamine B azide derivative.

Graphical abstract: Investigating the reactivities of a polyketide synthase module through fluorescent click chemistry

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Article information

DOI
https://doi.org/10.1039/C3CC47513A
Article type
Communication
Submitted
01 10 2013
Accepted
29 10 2013
First published
29 10 2013

Chem. Commun., 2014,50, 5276-5278

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Investigating the reactivities of a polyketide synthase module through fluorescent click chemistry

A. J. Hughes, M. R. Tibby, D. T. Wagner, J. N. Brantley and A. T. Keatinge-Clay, Chem. Commun., 2014, 50, 5276 DOI: 10.1039/C3CC47513A

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