A novel colorimetric assay for α-methylacyl-CoA racemase 1A (AMACR; P504S) utilizing the elimination of 2,4-dinitrophenolate

Maksims Yevglevskis; Guat L. Lee; Amit Nathubhai; Yoana D. Petrova; Tony D. James; Michael D. Threadgill; Timothy J. Woodman; Matthew D. Lloyd

doi:10.1039/C7CC00476A

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A novel colorimetric assay for α-methylacyl-CoA racemase 1A (AMACR; P504S) utilizing the elimination of 2,4-dinitrophenolate†

Maksims Yevglevskis,^a Guat L. Lee,^a Amit Nathubhai,^a Yoana D. Petrova,^a Tony D. James,

^b Michael D. Threadgill,^a Timothy J. Woodman^a and Matthew D. Lloyd

*^a

Author affiliations

* Corresponding authors

^a Drug & Target Development, Department of Pharmacy & Pharmacology, University of Bath, Claverton Down, Bath BA2 7AY, UK
E-mail: M.D.Lloyd@bath.ac.uk
Fax: +-44-1225-386114
Tel: +-44-1225-386786

^b Department of Chemistry, University of Bath, Claverton Down, Bath BA2 7AY, UK

Abstract

α-Methylacyl-CoA racemase (AMACR; P504S) regulates branched-chain fatty acid degradation, activates Ibuprofen and is a recognised cancer drug target. A novel, facile colorimetric assay was developed based on elimination of 2,4-dinitrophenolate. The assay was used to test 5 known inhibitors, determining IC₅₀ and K_i values, reversibility and characterizing irreversible inhibition.

This article is part of the themed collection: Chemosensors and Molecular Logic

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DOI: https://doi.org/10.1039/C7CC00476A
Article type: Communication
Submitted: 18 1 2017
Accepted: 17 3 2017
First published: 17 3 2017
This article is Open Access

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Chem. Commun., 2017,53, 5087-5090

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A novel colorimetric assay for α-methylacyl-CoA racemase 1A (AMACR; P504S) utilizing the elimination of 2,4-dinitrophenolate

M. Yevglevskis, G. L. Lee, A. Nathubhai, Y. D. Petrova, T. D. James, M. D. Threadgill, T. J. Woodman and M. D. Lloyd, Chem. Commun., 2017, 53, 5087 DOI: 10.1039/C7CC00476A

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Chemical Communications

A novel colorimetric assay for α-methylacyl-CoA racemase 1A (AMACR; P504S) utilizing the elimination of 2,4-dinitrophenolate†

Abstract

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A novel colorimetric assay for α-methylacyl-CoA racemase 1A (AMACR; P504S) utilizing the elimination of 2,4-dinitrophenolate

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