Issue 8, 2017

Imaging drug uptake by bioorthogonal stimulated Raman scattering microscopy

Abstract

Stimulated Raman scattering (SRS) microscopy in tandem with bioorthogonal Raman labelling strategies is set to revolutionise the direct visualisation of intracellular drug uptake. Rational evaluation of a series of Raman-active labels has allowed the identification of highly active labels which have minimal perturbation on the biological efficacy of the parent drug. Drug uptake has been correlated with markers of cellular composition and cell cycle status, and mapped across intracellular structures using dual-colour and multi-modal imaging. The minimal phototoxicity and low photobleaching associated with SRS microscopy has enabled real-time imaging in live cells. These studies demonstrate the potential for SRS microscopy in the drug development process.

Graphical abstract: Imaging drug uptake by bioorthogonal stimulated Raman scattering microscopy

Supplementary files

Article information

Article type
Edge Article
Submitted
25 4 2017
Accepted
15 5 2017
First published
24 5 2017
This article is Open Access

All publication charges for this article have been paid for by the Royal Society of Chemistry
Creative Commons BY license

Chem. Sci., 2017,8, 5606-5615

Imaging drug uptake by bioorthogonal stimulated Raman scattering microscopy

W. J. Tipping, M. Lee, A. Serrels, V. G. Brunton and A. N. Hulme, Chem. Sci., 2017, 8, 5606 DOI: 10.1039/C7SC01837A

This article is licensed under a Creative Commons Attribution 3.0 Unported Licence. You can use material from this article in other publications without requesting further permissions from the RSC, provided that the correct acknowledgement is given.

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