A far-red fluorescent probe for sensing laccase in fungi and its application in developing an effective biocatalyst for the biosynthesis of antituberculous dicoumarin

Zhenhao Tian; Qingsong Yan; Lei Feng; Sa Deng; Chao Wang; Jingnan Cui; Chunjie Wang; Zhanjun Zhang; Tony D. James; Xiaochi Ma

doi:10.1039/C9CC01579E

A far-red fluorescent probe for sensing laccase in fungi and its application in developing an effective biocatalyst for the biosynthesis of antituberculous dicoumarin†

Zhenhao Tian,‡^ab Qingsong Yan,‡^a Lei Feng,

‡^abc Sa Deng,^a Chao Wang,

*^ab Jingnan Cui,^b Chunjie Wang,^a Zhanjun Zhang,^d Tony D. James

*^c and Xiaochi Ma

*^a

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* Corresponding authors

^a National-Local Joint Engineering Research Center for Drug-Research and Development of Neurodegenerative Disease, Academy of Integrative Medicine, College of Pharmacy, Dalian Medical University, Dalian, China
E-mail: wach_edu@sina.com, maxc1978@163.com

^b State Key Laboratory of Fine Chemicals, Dalian University of Technology, Dalian 116024, China

^c Department of Chemistry, University of Bath, Bath BA2 7AY, UK
E-mail: t.d.james@bath.ac.uk

^d State Key Laboratory of Cognitive Neuroscience and Learning, Beijing Normal University, Beijing, China

Abstract

A far-red fluorescent probe has been developed for sensing fungal laccase. The probe was used to determine that Rhizopus oryzae had a high level endogenous laccase amongst 24 fungal strains. The Rhizopus oryzae was then used as a biocatalyst for the preparation of dicoumarin resulting in significant inhibition of Mycobacterium tuberculosis H37Ra.

This article is part of the themed collection: The Mechanics of Supramolecular Chemistry

Chemical Communications

A far-red fluorescent probe for sensing laccase in fungi and its application in developing an effective biocatalyst for the biosynthesis of antituberculous dicoumarin†

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A far-red fluorescent probe for sensing laccase in fungi and its application in developing an effective biocatalyst for the biosynthesis of antituberculous dicoumarin

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