Issue 28, 2019

Dual resonance energy transfer in triple-component polymer dots to enhance electrochemiluminescence for highly sensitive bioanalysis

Abstract

Polymer dots (Pdots) have become a type of attractive illuminant for electrochemiluminescence (ECL). However, the low ECL efficiency severely limits their practicability. Here, we design a dual intramolecular resonance energy transfer (RET) mechanism with newly synthesized triple-component Pdots to achieve great ECL enhancement. This mechanism efficiently shortens the path of energy transmission, thus greatly promoting the ECL amplification by 380 and 31 times compared to systems with no and single RET, and results in a relative ECL efficiency of 23.1% (vs. 1 mM Ru(bpy)32+). Using metal–organic frameworks to carry the triple-component Pdots, a highly luminescent probe is proposed. By integrating the probe with target-mediated enzymatic circulation amplification and DNA arrays, a highly sensitive ECL imaging method is designed for simultaneous visual analysis of two kinds of proteins, mucin 1 and human epidermal growth factor receptor 2, on living cells, which exhibited linear ranges of 1 pg mL−1 to 5 ng mL−1 and 5 pg mL−1 to 10 ng mL−1 with limits of detection of 1 pg mL−1 and 5 pg mL−1, respectively. The proposed strategy showed promising application in bioanalysis.

Graphical abstract: Dual resonance energy transfer in triple-component polymer dots to enhance electrochemiluminescence for highly sensitive bioanalysis

Supplementary files

Article information

Article type
Edge Article
Submitted
31 3 2019
Accepted
28 5 2019
First published
30 5 2019
This article is Open Access

All publication charges for this article have been paid for by the Royal Society of Chemistry
Creative Commons BY license

Chem. Sci., 2019,10, 6815-6820

Dual resonance energy transfer in triple-component polymer dots to enhance electrochemiluminescence for highly sensitive bioanalysis

N. Wang, Z. Wang, L. Chen, W. Chen, Y. Quan, Y. Cheng and H. Ju, Chem. Sci., 2019, 10, 6815 DOI: 10.1039/C9SC01570A

This article is licensed under a Creative Commons Attribution 3.0 Unported Licence. You can use material from this article in other publications without requesting further permissions from the RSC, provided that the correct acknowledgement is given.

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