Issue 5, 2022

Structural impact of thioamide incorporation into a β-hairpin

Abstract

The thioamide is a naturally-occurring single atom substitution of the canonical amide bond. The exchange of oxygen to sulfur alters the amide's physical and chemical characteristics, thereby expanding its functionality. Incorporation of thioamides in prevalent secondary structures has demonstrated that they can either have stabilizing, destabilizing, or neutral effects. We performed a systematic investigation of the structural impact of thioamide incorporation in a β-hairpin scaffold with nuclear magnetic resonance (NMR). Thioamides as hydrogen bond donors did not increase the foldedness of the more stable “YKL” variant of this scaffold. In the less stable “HPT” variant of the scaffold, the thioamide could be stabilizing as a hydrogen bond donor and destabilizing as a hydrogen bond acceptor, but the extent of the perturbation depended upon the position of incorporation. To better understand these effects we performed structural modelling of the macrocyclic folded HPT variants. Finally, we compare the thioamide effects that we observe to previous studies of both side-chain and backbone perturbations to this β-hairpin scaffold to provide context for our observations.

Graphical abstract: Structural impact of thioamide incorporation into a β-hairpin

Supplementary files

Article information

Article type
Paper
Submitted
29 11 2021
Accepted
24 3 2022
First published
05 4 2022
This article is Open Access
Creative Commons BY license

RSC Chem. Biol., 2022,3, 582-591

Structural impact of thioamide incorporation into a β-hairpin

K. E. Fiore, M. J. Patist, S. Giannakoulias, C. Huang, H. Verma, B. Khatri, R. P. Cheng, J. Chatterjee and E. J. Petersson, RSC Chem. Biol., 2022, 3, 582 DOI: 10.1039/D1CB00229E

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