Monitoring mitochondrial nitroreductase activity in tumors and a hind-limb model of ischemia in mice using a novel activatable NIR fluorescent probe

Xiaosheng Liu; Shuang Zeng; Ming Zhang; Maojun Jiang; Yves S. Kafuti; Pingping Shangguan; Yichu Yu; Qixian Chen; Jingyun Wang; Xiaojun Peng; Juyoung Yoon; Haidong Li

doi:10.1039/D2CC04112J

Monitoring mitochondrial nitroreductase activity in tumors and a hind-limb model of ischemia in mice using a novel activatable NIR fluorescent probe†

Xiaosheng Liu,‡^a Shuang Zeng,‡^a Ming Zhang,^a Maojun Jiang,^b Yves S. Kafuti,^a Pingping Shangguan,^a Yichu Yu,^a Qixian Chen,

^a Jingyun Wang,

*^ab Xiaojun Peng,

^b Juyoung Yoon

*^c and Haidong Li

*^ab

Author affiliations

* Corresponding authors

^a School of Bioengineering, Dalian University of Technology, 2 Linggong Road, Hi-tech Zone, Dalian 116024, China
E-mail: wangjingyun67@dlut.edu.cn, lihd@dlut.edu.cn

^b State Key Laboratory of Fine Chemicals, Frontiers Science Center for Smart Materials Oriented Chemical Engineering, Dalian University of Technology, 2 Linggong Road, Hi-tech Zone, Dalian 116024, China

^c Department of Chemistry and Nanoscience, Ewha Womans University, 52 Ewhayeodae-gil, Seodaemun-gu, Seoul 03760, Korea
E-mail: jyoon@ewha.ac.kr

Abstract

We report a mitochondria-targeted nitroreductase (NTR)-activated near-infrared fluorescent probe: CS-NO₂. Overexpressed NTR in mitochondria was measured with high sensitivity. More importantly, the probe CS-NO₂ successfully monitored NTR activity in solid tumors and a hind-limb model of ischemia in mice. This novel finding indicates the promising function of our probe for the diagnosis of solid tumors and hypoxia-associated diseases.

This article is part of the themed collection: Sensors for Health

Chemical Communications

Monitoring mitochondrial nitroreductase activity in tumors and a hind-limb model of ischemia in mice using a novel activatable NIR fluorescent probe†

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Monitoring mitochondrial nitroreductase activity in tumors and a hind-limb model of ischemia in mice using a novel activatable NIR fluorescent probe

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