Issue 16, 2023

Protruding cantilever microelectrode array to monitor the inner electrical activity of cerebral organoids

Abstract

Stem cell-derived cerebral organoids are artificially grown miniature organ-like structures mimicking embryonic brain architecture. They are composed of multiple neural cell types with 3D cell layer organization exhibiting local field potential. Measuring the extracellular electrical activity by means of conventional planar microelectrode arrays is particularly challenging due to the 3D architecture of organoids. In order to monitor the intra-organoid electrical activity of thick spheroid-shaped samples, we developed long protruding microelectrode arrays able to penetrate the inner regions of cerebral organoids to measure the local potential of neurons within the organoids. A new microfabrication process has been developed which, thanks to the relaxation of internal stresses of a stack of materials deposited over a sacrificial layer, allows one to build a protruding cantilever microelectrode array placed at the apex of beams which rise vertically, over two hundred microns. These slender beams inserted deeply into the organoids give access to the recording of local field potential from neurons buried inside the organoid. This novel device shall provide valuable tools to study neural functions in greater detail.

Graphical abstract: Protruding cantilever microelectrode array to monitor the inner electrical activity of cerebral organoids

Supplementary files

Article information

Article type
Paper
Submitted
05 4 2023
Accepted
14 7 2023
First published
17 7 2023
This article is Open Access
Creative Commons BY-NC license

Lab Chip, 2023,23, 3603-3614

Protruding cantilever microelectrode array to monitor the inner electrical activity of cerebral organoids

O. Phouphetlinthong, E. Partiot, C. Bernou, A. Sebban, R. Gaudin and B. Charlot, Lab Chip, 2023, 23, 3603 DOI: 10.1039/D3LC00294B

This article is licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported Licence. You can use material from this article in other publications, without requesting further permission from the RSC, provided that the correct acknowledgement is given and it is not used for commercial purposes.

To request permission to reproduce material from this article in a commercial publication, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party commercial publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements