Issue 9, 2024

Constructing a green oleaginous yeast cell factory for sustainable production of the plant-derived diterpenoid sclareol

Abstract

Diterpenoids are an important class of secondary metabolites in nature with diverse applications such as pharmaceuticals, flavors and fragrances. Sclareol, a plant-derived diterpene alcohol from Salvia sclarea, is most commonly used as the starting material for the production of hemisynthetic Ambrox, a valuable substitute for ambergris from sperm whales used in perfumery. Here, we engineered the oleaginous yeast Yarrowia lipolytica as a chassis for sclareol overproduction. We constructed a de novo sclareol biosynthetic pathway by screening and engineering heterologous LPP synthase (Lpps) and sclareol synthase (Scs). By constructing a two-layer geranylgeranyl diphosphate (GGPP) accumulation pathway and scaffold-free multienzyme complexes, the metabolic flux was redirected toward sclareol synthesis, enabling significantly improved production of sclareol and reduced levels of by-products. The engineered strain was capable of producing 12.9 g Lāˆ’1 of sclareol in a 5 L bioreactor, with this being the highest titer achieved to date. To the best of our knowledge, this is the first report of sclareol biosynthesis in Y. lipolytica, and this chassis strain may offer a groundbreaking foundation for the sustainable, large-scale fermentation of diterpenoids.

Graphical abstract: Constructing a green oleaginous yeast cell factory for sustainable production of the plant-derived diterpenoid sclareol

Supplementary files

Article information

Article type
Paper
Submitted
14 12 2023
Accepted
12 2 2024
First published
15 2 2024

Green Chem., 2024,26, 5202-5210

Constructing a green oleaginous yeast cell factory for sustainable production of the plant-derived diterpenoid sclareol

M. Sun, Y. Han, X. Yu, K. Wang, L. Lin, R. Ledesma-Amaro and X. Ji, Green Chem., 2024, 26, 5202 DOI: 10.1039/D3GC04949C

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