Issue 9, 2023

Facilitation of axonal transcriptome analysis with quantitative microfluidic devices

Abstract

Microfluidic chambers are powerful tools for studying axonal mRNA localization and translation in neurons. In addition to specific manipulation and measurements of axons, microfluidic chambers are used for collecting axonal materials to perform axonal transcriptome analysis. However, traditional bipartite and tripartite chambers have limitations either in purity or quantity of collected axons. Here, we improved the design of traditional chambers. Moreover, we developed two new quantitative chambers, multi-compartmental quantitative bipartite chamber (MQBC) and long quantitative tripartite chamber (LQTC). Compared with the traditional chambers, MQBC and LQTC could dramatically increase the efficiency in collecting axonal RNA. Finally, we applied these chambers to do comparative axon transcriptome analysis of different types of neurons. Thus, our newly designed quantitative chambers significantly improve axon collection efficiency and facilitate axonal transcriptome analysis.

Graphical abstract: Facilitation of axonal transcriptome analysis with quantitative microfluidic devices

Supplementary files

Article information

Article type
Paper
Submitted
27 12 2022
Accepted
04 4 2023
First published
05 4 2023

Lab Chip, 2023,23, 2217-2227

Facilitation of axonal transcriptome analysis with quantitative microfluidic devices

Z. Yang, J. Yu, J. Zhang, H. Song, H. Ye, J. Liu, N. Wang, P. Che, G. Long, Y. Wang, J. Park and S. Ji, Lab Chip, 2023, 23, 2217 DOI: 10.1039/D2LC01183B

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