Themed collection Analyst Recent HOT articles

Nanopore electrochemistry, as one of the promising tools for single molecule sensing, has proved its capability in DNA sequencing and protein analysis.

Currently, there exists a multitude of different exosome isolation and characterization approaches based on biophysical and/or biochemical characteristics. Herein we review various technologies for separation of distinct exosome subpopulations.

Traditional enzymology relies on the kinetics of millions of enzymes, an experimental approach that may wash out heterogeneities between individual enzymes. Electrochemical methods have emerged in the last 5 years to probe single enzyme reactivity.

Recent advancements regarding the application of MIPs and relevant virus imprinting techniques for the detection of viruses are highlighted.

Self-assembled nanomaterials (SANs) exhibit designable biofunctions owing to their tunable nanostructures and modifiable surfaces.

The utilization of the DNA origami technique in the fabrication of complex objects has given rise to possibilities in DNA-induced precise manufacturing and might open a new era of bottom-up fabrication.

Molecular biology and nanotechnology based analytical methods have proven to be promising diagnostic tools for the rapid diagnosis of COVID-19.

Gas-propelled biosensors display a simple gas-based signal amplification with quantitative detection features based on the target recognition event in combination with gas propulsion.

Stimulated Raman histopathology with computer aided diagnosis.

Classification of nanoclusters and methods to improve their quantum yield and applications.

This review discusses the recent developments in microfluidics technology for antimicrobial susceptibility testing with a special emphasis on the nucleic acid amplification-based approach.

This tutorial review provides an overview of key concepts in metabolomics and their application to develop successful strategies in regulatory toxicology-oriented studies.

In this review, the research progress of the sensors based on the DNA-nanozymes were summarized.

The review describes the design, application and performance of current smartphone-based colorimeters, photo- and spectrometers and fluorimeters. Furthermore, it gives an overview of the advantages and disadvantages of such systems.

Schematic of two main research directions of lateral flow assays for nucleic acid detection.

This review presents an overview of current analytical trends in antibody characterization by multidimensional LC-MS approaches.

Changes in the electrical conductance of graphene field-effect transistors (GFETs) are used to perform quantitative analyses of biologically-relevant molecules such as DNA, proteins, ions and small molecules.

A simple target-responsive CRISPR/Cas12a-based strategy for translating molecular detection into a glucose test.

A three-channel ratiometric ECL platform for MCF-7 cells detection using potential-resolved ECL probes.

4-Mercaptobenzoic acid was first introduced as a MALDI matrix for metal analysis. The developed method was successfully applied to the rapid screening and sensitive determination of metals in PM_2.5 samples.

A series of fluorescent molecular rotors, acridinium benzoates, were designed for ratiometrically monitoring cellular viscosity with high sensitivity.

“Signal-on” photoelectrochemical biofuel cell-based self-powered biosensors based on metal–organic framework-controlled release behavior were constructed for ultrasensitive microRNA detection.

Potassium-selective polymer dots (K-Pdots) containing potassium ionophores were for the first time used for photoelectrochemical (PEC) analysis and yielded sensitive and specific detection of potassium ions.

This paper describes the detection of endogenous cyanide using corrin-based CyanoKit technologies in combination with a smartphone readout device.

We present a smartphone-based portable Raman spectrometer, which has a friendly human–machine interface, easy operation, rapid response time, and most importantly a very small size for on-site use.

Lectin inspired polymers polySD-SiO₂ were prepared and applied to the high-efficiency enrichment of glycopeptides.

Employing the redox species N,N,N′,N′-tetramethyl-para-phenylene-diamine (TMPD), the label-free detection of E. coli, based on an electrochemical “on”-signal during impact electrochemistry, is reported for the first time.

We develop a ratiometric Raman probe for visualizing hydrogen sulfide in living cells as the first alkyne-based sensor for SRS microscopy.

We describe a label-free approach based on Raman spectroscopy, to study drug-induced apoptosis in vivo.

This paper presents the first demonstration of highly sensitive and on-site SERS-based NO₂ sensors with a detection limit of 0.1 ppm operated under ambient conditions.

A new immunoassay was designed for the detection of disease-related biomarkers (alpha-fetoprotein, AFP, as a model), coupling an aluminium (Al)/Prussian blue-based electrochromic display with a digital multimeter readout.

A quantified POCT device was designed based on wettability alteration in a capillary tube.

The mechanism of a novel electrochemiluminescence biosensor based on S-doped yttrium oxide ultrathin nanosheets for detection of anti-Dig antibodies.

We showed that molecularly imprinted nanoparticles (nanoMIPs) could efficiently inhibit the activities of the RNase in RT-PCR reactions, demonstrating that the tailor-made nanomaterials are very promising for use in routine biological assays.

A homogeneous assay for proteins and small molecules with ultra-high inhibition factors and sensitivity was established.

A thumb-size and precise electrochemical system is designed for sensors in size and cost sensitive applications.

An ultrasensitive PEC bioanalysis strategy was designed based on the significantly enhanced signal of BiOI/g-C₃N₄ and amplified signal variation of CuS NPs.

Plasmonic gold nanorods are promising and sensitive light scattering probes, which can reach the single particle level. Herein, we present the scattering properties of gold nanorods for time-resolved visual detection of heparin based on the rapid etching of gold nanorods under dark-field microscopy.

With improved deconvolution and fragment identification strategies, we use the patterns of specific modifications of fragment ions resulted from disulfide cleavage in top-down mass spectrometry to deduce the complex disulfide patterns of cysteine-rich proteins.

The interactions between NPs and a binary system of proteins were studied through a systematic set of capillary electrophoresis models. The results revealed precise details of the soft and hard protein corona formation and dynamics.

Several aptamers specific to ESCC cells were successfully screened using cell-SELEX, and a truncated ultrashort aptamer was found suitable for imaging ESCC tissues of patients.

Combination of experimentally determined collision-cross sections provided by trapped ion mobility high-resolution mass spectrometry with in-silico calculations for structural assessment of petroporphyrins within an ultra-complex crude oil matrix.

A sensitive electrochemical immunosensor based on a bienzymatic system consisting of β-galactosidase and flavin adenine dinucleotide-dependent glucose dehydrogenase was developed.

Oral bioaccessibility of plastic migrants using automatic flow methodology.

A simple, sensitive method based on microplasma-induced chemical vapor generation for the rapid screening and quantification of mercury in fruits and vegetables by atomic spectrometry without sample preparation.

A broad-specific anti-B[a]P mAb (4E8) was prepared with IC₅₀ value of 2.51 ng mL⁻¹. The GNP-ICS assay showed broad specificity in the detection of B[a]P and its analogues, with cut-off and vLOD values of 100 and 10 ng mL⁻¹, respectively.

TiN nanopillar was applied as a robust analytical substrate for the laser desorption/ionization mass spectrometry of various types of analytes with a wide mass range. Numerical simulation validated the heat confinement effect of the pillar shape.

We developed a novel fast gas chromatography (fastGC) instrument with integrated sampling of volatile organic compounds (VOCs) and detection by single-photon ionisation (SPI) time-of-flight mass spectrometry (TOFMS).

A thin film molecularly imprinted polymer (TF-MIP) is developed for isolation of tricyclic antidepressants (TCAs). This single use microextraction device is applied for selective and high-throughput extraction of TCAs from biological matrices.

A new ambient ionization method, 3D-printed cone spray ionization mass spectrometry (3D-PCSI-MS), is implemented for rapid profiling of chemical warfare agent (CWA) simulants in unprepared soil samples.

An HIV detection assay featuring a low-cost μPAD for electrophoretic RNA extraction and rapid isothermal nucleic acid amplification.

Based on the stimulus-responsive lanthanide coordination polymer and the stable fluorescence of carbon dots (CDs), a simple ratiometric fluorescence sensing strategy was proposed to detect ALP activity.

The identification of Justicia spicigera in the Codex Borbonicus by Raman spectroscopy motivated the development of a multi-analytical techniques approach to broaden the physico-chemical knowledge regarding this traditional dye source.

A smartphone-based fluorescent microscope capable of imaging multiple fluorescent probes at different magnification levels.

A novel dual cavity UV-VIS spectrometer for sensitive monitoring of urban NO_x and other interfering pollutants.

Compared with the common G-quadruplex-based molecular beacon, the G-triplex-based molecular beacon exhibits higher sensitivity and faster response speed.

A workflow for bacterial pathogen enrichment and DNA extraction and isolation in the single-tube sample preparation method for nucleic acid-based detection of nontyphoidal salmonella is presented.

The first 5-fluorouracil-nutraceutical cocrystal with synergistic antitumor effect, Flu-FR-H₂O, is synthesized based on the dual optimization strategy and evaluated by combining the experimental evaluation with theoretical analysis.

Microdroplet mass spectrometry for the location of carbon–carbon double bonds in unsaturated fatty acids and lipids via ROS generation at the air–water interface.

Deep learning models are developed to identify the silk proteins and evaluate their relative content of each conformation from FTIR spectra. These models are highly accurate and time-efficient and promise to process massive FTIR data sets.

A new strategy for in situ and simultaneously monitoring endogenous MAPK kinases in single living cells based on a multi-channel FCS system and affinity fluorescent probes is developed.

Dissociation of large multiply protonated serine clusters produces abundant protonated octamer at an optimum ion temperature predominantly through a doubly protonated decamer intermediate.

An electrochemical sensor based on a 3D cobalt-based oxide modified boron and nitrogen co-doped graphene hydrogel exhibited excellent sensitivity and catalytic induction properties.

Complete indirect antiglobulin test (IAT) in a single diagnostic incorporating a laser-incubated droplet on a hydrophobic surface with read-out provided by a paper strip.

We designed DNA nanolantern-based split aptamer nanoprobes for in situ ratiometric analysis of ATP expression in living cells.

A metal–organic framework with multienzyme activity was designed as an electrochemical material for real-time electrochemical detection of nitric oxide and hydrogen peroxide.

A fully automated novel workflow for lipidomics based on flow injection, followed by reversed phase liquid chromatography–high-resolution mass spectrometry in combination with LILY as internal standard, enabling accurate quantification via RP-LC.

To selectively detect H₂S, a novel dual-channel fluorescence probe, triphenylamine piperazine NBD (TPA-Pz-NBD), was developed based on the thiolysis reaction of NBD amine.

As inflammation plays a role in the pathogenesis of acute coronary syndromes, C-reactive protein (CRP) can be used as a biomarker. The present study constructed the electrochemcial biosensor with nanobio hybrid material for CRP detection.

Analyzing the SOD-like activity of nanozymes in vitro is of great importance for identifying new nanozymes and predicting their potential biological effects in vivo.