The role of cysteine and sulfide in the interplay between microbial Hg(ii) uptake and sulfur metabolism

Abstract

Biogenic thiols, such as cysteine, have been used to control the speciation of Hg(II) in bacterial exposure experiments. However, the extracellular biodegradation of excess cysteine leads to the formation of Hg(II)–sulfide species, convoluting the interpretation of Hg(II) uptake results. Herein, we test the hypothesis that Hg(II)–sulfide species formation is a critical step during bacterial Hg(II) uptake in the presence of excess cysteine. An Escherichia coli (E. coli) wild-type and mutant strain lacking the decR gene that regulates cysteine degradation to sulfide were exposed to 50 and 500 nM Hg with 0 to 2 mM cysteine. The decR mutant released ∼4 times less sulfide from cysteine degradation compared to the wild-type for all tested cysteine concentrations during a 3 hour exposure period. We show with thermodynamic calculations that the predicted concentration of Hg(II)–cysteine species remaining in the exposure medium (as opposed to forming HgS_(s)) is a good proxy for the measured concentration of dissolved Hg(II) (i.e., not cell-bound). Likewise, the measured cell-bound Hg(II) correlates with thermodynamic calculations for HgS_(s) formation in the presence of cysteine. High resolution X-ray absorption near edge structure (HR-XANES) spectra confirm the existence of cell-associated HgS_(s) at 500 nM total Hg and suggest the formation of Hg–S clusters at 50 nM total Hg. Our results indicate that a speciation change to Hg(II)–sulfide controls Hg(II) cell-association in the presence of excess cysteine.