Posttranscriptional spin labeling of RNA by tetrazine-based cycloaddition†
Olav
Schiemann 
b
and
Stephanie
Kath-Schorr
*a
b
and
Stephanie
Kath-Schorr
*a
Abstract
The site-specific introduction of spin labels into RNA for distance measurements by EPR gives insight into its solution structure. We here present a method for spin labeling of in vitro transcribed RNA. Distance distributions between two nitroxide spin labels are determined by PELDOR in a self-complementary RNA duplex.
- This article is part of the themed collections: Chemical Biology in OBC and New Talent
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