Detection of the effect of polydopamine (PDA)-coated polydimethylsiloxane (PDMS) substrates on the release of H2O2 from a single HeLa cell

Jingyu Xiao; Meng Tian; Lei Su; Yu Bao; Li Niu; Xueji Zhang

doi:10.1039/D1AN01506K

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Detection of the effect of polydopamine (PDA)-coated polydimethylsiloxane (PDMS) substrates on the release of H₂O₂ from a single HeLa cell†

Jingyu Xiao,^b Meng Tian,^b Lei Su,

*^a Yu Bao,*^c Li Niu

^c and Xueji Zhang

*^a

Author affiliations

* Corresponding authors

^a School of Biomedical Engineering, Health Science Center, Shenzhen University, Shenzhen 518037, P.R. China, P. R. China
E-mail: sulei@szu.edu.cn, zhangxueji@szu.edu.cn

^b School of Chemistry and Biological Engineering, University of Science and Technology Beijing, Beijing 100083, P. R. China

^c Center for Advanced Analytical Science, School of Chemistry and Chemical Engineering, Guangzhou University, Guangzhou 510006, P. R. China
E-mail: baoyu@gzhu.edu.cn

Abstract

Endogenous H₂O₂ generated by a single HeLa cell that was adhered on the PDA-coated PDMS substrates under 25 mM glucose culture conditions was detected using a home-built photoelectric dual detection platform. With PMA as the stimulus, the cell released a small amount of H₂O₂ and its mitochondrial membrane potential (MMP) decrease was smaller, compared with that on the PDMS substrates.

This article is part of the themed collection: Analyst HOT Articles 2021

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Article information

DOI: https://doi.org/10.1039/D1AN01506K
Article type: Communication
Submitted: 19 Aug. 2021
Accepted: 07 Sept. 2021
First published: 08 Sept. 2021

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Analyst, 2021,146, 6445-6449

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Detection of the effect of polydopamine (PDA)-coated polydimethylsiloxane (PDMS) substrates on the release of H₂O₂ from a single HeLa cell

J. Xiao, M. Tian, L. Su, Y. Bao, L. Niu and X. Zhang, Analyst, 2021, 146, 6445 DOI: 10.1039/D1AN01506K

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