Immobilization of an artificial imine reductase within silica nanoparticles improves its performance

Martina Hestericová; M. Rita Correro; Markus Lenz; Philippe F.-X. Corvini; Patrick Shahgaldian; Thomas R. Ward

doi:10.1039/C6CC04604E

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Immobilization of an artificial imine reductase within silica nanoparticles improves its performance†

Martina Hestericová,^a M. Rita Correro,^b Markus Lenz,^c Philippe F.-X. Corvini,^c Patrick Shahgaldian*^b and Thomas R. Ward*^a

Author affiliations

* Corresponding authors

^a Department of Chemistry, University of Basel, Spitalstrasse 51, CH-4056 Basel, Switzerland
E-mail: thomas.ward@unibas.ch

^b Institute of Chemistry and Bioanalytics, School of Life Sciences, University of Applied Sciences and Arts Northwestern Switzerland, Gründenstrasse 40, CH-4132 Muttenz, Switzerland

^c Institute for Ecopreneurship, School of Life Sciences, University of Applied Sciences and Arts Northwestern Switzerland, Gründenstrasse 40, 4132 Muttenz, Switzerland

Abstract

Silica nanoparticles equipped with an artificial imine reductase display remarkable activity towards cyclic imine- and NAD⁺ reduction. The method, based on immobilization and protection of streptavidin on silica nanoparticles, shields the biotinylated metal cofactor against deactivation yielding over 46 000 turnovers in pure samples and 4000 turnovers in crude cellular extracts.

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Article information

DOI: https://doi.org/10.1039/C6CC04604E
Article type: Communication
Submitted: 01 Jūn. 2016
Accepted: 28 Jūn. 2016
First published: 06 Jūl. 2016
This article is Open Access

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Chem. Commun., 2016,52, 9462-9465

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Immobilization of an artificial imine reductase within silica nanoparticles improves its performance

M. Hestericová, M. R. Correro, M. Lenz, P. F.-X. Corvini, P. Shahgaldian and T. R. Ward, Chem. Commun., 2016, 52, 9462 DOI: 10.1039/C6CC04604E

This article is licensed under a Creative Commons Attribution 3.0 Unported Licence. You can use material from this article in other publications without requesting further permissions from the RSC, provided that the correct acknowledgement is given.

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Chemical Communications

Immobilization of an artificial imine reductase within silica nanoparticles improves its performance†

Abstract

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Immobilization of an artificial imine reductase within silica nanoparticles improves its performance

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