Dual-labeling of ubiquitin proteins by chemoselective reactions for sensing UCH-L3

Lei Li; Si-Yu Zhang; Yan-Mei Li; Yong-Xiang Chen

doi:10.1039/C6MB00165C

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Dual-labeling of ubiquitin proteins by chemoselective reactions for sensing UCH-L3†

Lei Li,^a Si-Yu Zhang,^a Yan-Mei Li^a and Yong-Xiang Chen*^a

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* Corresponding authors

^a Key Laboratory of Bioorganic Phosphorus Chemistry & Chemical Biology (Ministry of Education), Department of Chemistry, Tsinghua University, Beijing 100084, P. R. China
E-mail: chen-yx@mail.tsinghua.edu.cn
Fax: +86-10-62781695
Tel: +86-10-62772259

Abstract

A site-specific dual-color labeled ubiquitin for sensing deubiquitinase's activity was prepared by consecutively using chemoselective native chemical ligation reactions in a facile and efficient way. The prepared sensor was applied to establish a sensitive FRET-based assay for UCH-L3.

This article is part of the themed collections: Chemical Biology in Molecular BioSystems and Protein Labelling

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Article information

DOI: https://doi.org/10.1039/C6MB00165C
Article type: Communication
Submitted: 03 Marts 2016
Accepted: 13 Apr. 2016
First published: 13 Apr. 2016

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Mol. BioSyst., 2016,12, 1764-1767

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Dual-labeling of ubiquitin proteins by chemoselective reactions for sensing UCH-L3

L. Li, S. Zhang, Y. Li and Y. Chen, Mol. BioSyst., 2016, 12, 1764 DOI: 10.1039/C6MB00165C

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Molecular BioSystems

Dual-labeling of ubiquitin proteins by chemoselective reactions for sensing UCH-L3†

Abstract

Supplementary files

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Dual-labeling of ubiquitin proteins by chemoselective reactions for sensing UCH-L3

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