Development of a reversible fluorescent probe for reactive sulfur species, sulfane sulfur, and its biological application

Yoko Takano; Kenjiro Hanaoka; Kazuhito Shimamoto; Ryo Miyamoto; Toru Komatsu; Tasuku Ueno; Takuya Terai; Hideo Kimura; Tetsuo Nagano; Yasuteru Urano

doi:10.1039/C6CC08372B

Development of a reversible fluorescent probe for reactive sulfur species, sulfane sulfur, and its biological application†

Yoko Takano,

^a Kenjiro Hanaoka,*^a Kazuhito Shimamoto,^a Ryo Miyamoto,^b Toru Komatsu,^ac Tasuku Ueno,^a Takuya Terai,

^a Hideo Kimura,^b Tetsuo Nagano^d and Yasuteru Urano*^aef

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* Corresponding authors

^a Graduate School of Pharmaceutical Sciences, The University of Tokyo, 7-3-1, Hongo, Bunkyo-ku, Tokyo 113-0033, Japan
E-mail: uranokun@m.u-tokyo.ac.jp, khanaoka@mol.f.u-tokyo.ac.jp

^b Department of Molecular Pharmacology, National Institute of Neuroscience, National Center of Neurology and Psychiatry, 4-1-1 Ogawa-Higashi, Kodaira, Tokyo 187-8502, Japan

^c PRESTO, Japan Science and Technology Agency (JST), 4-1-8 Honcho, Kawaguchi, Saitama 332-0012, Japan

^d Drug Discovery Initiative, The University of Tokyo, 7-3-1, Hongo, Bunkyo-ku, Tokyo 113-0033, Japan

^e Graduate School of Medicine, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan

^f AMED CREST (Japan) Agency for Medical Research and Development 1-7-1 Otemachi, Chiyoda-ku, Tokyo 100-0004, Japan

Abstract

We report a reversible off/on fluorescent probe for monitoring concentration changes of sulfane sulfur by utilizing the unique ability of sulfane sulfur to bind reversibly to other sulfur atoms and the intramolecular spirocyclization reaction of xanthene dyes. It reversibly visualized sulfane sulfur in living A549 cells and primary-cultured hippocampal astrocytes.

This article is part of the themed collections: RSC papers by GRC Bioanalytical Sensors 2018 Speakers and Most downloaded articles of 2017: Analytical Chemistry

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Development of a reversible fluorescent probe for reactive sulfur species, sulfane sulfur, and its biological application†

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Development of a reversible fluorescent probe for reactive sulfur species, sulfane sulfur, and its biological application

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