Lanthanide-based peptide biosensor to monitor CDK4/cyclin D kinase activity†
M.
Eugenio Vázquez 
b
and
May C.
Morris
*a
b
and
May C.
Morris
*a
Abstract
We describe a lanthanide biosensor that responds to CDK4 kinase activity in melanoma cell extracts through a significant and dose dependent increase in luminescence, thanks to sensitization of a DOTA[Tb3+] complex incorporated into a CDK4 substrate peptide by a unique tryptophan residue in an adjacent phosphoaminoacid binding moiety.
- This article is part of the themed collection: Biological optical imaging
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