ortho-Fluoroazobenzene derivatives as DNA intercalators for photocontrol of DNA and nucleosome binding by visible light

Benedikt Heinrich; Karim Bouazoune; Matthias Wojcik; Udo Bakowsky; Olalla Vázquez

doi:10.1039/C8OB02343C

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ortho-Fluoroazobenzene derivatives as DNA intercalators for photocontrol of DNA and nucleosome binding by visible light†

Benedikt Heinrich,

^a Karim Bouazoune,

^b Matthias Wojcik,

^c Udo Bakowsky

^c and Olalla Vázquez

*^a

Author affiliations

* Corresponding authors

^a Fachbereich Chemie, Philipps-Universität Marburg, Hans-Meerwein-Straße 4, Marburg, Germany
E-mail: olalla.vazquez@staff.uni-maburg.de

^b Institut für Molekularbiologie und Tumorforschung, Philipps-Universität Marburg, Hans-Meerwein-Straße 2, Marburg, Germany

^c Institut für Pharmazeutische Technologie & Biopharmazie, Philipps-Universität Marburg, Robert-Koch-Straße 4, Marburg, Germany

Abstract

We report a high-affinity photoswitchable DNA binder, which displays different nucleosome-binding capacities upon visible-light irradiation. Both photochemical and DNA-recognition properties were examined by UV-Vis, HPLC, CD spectroscopy, NMR, FID assays, EMSA and DLS. Our probe sets the basis for developing new optoepigenetic tools for conditional modulation of nucleosomal DNA accessibility.

This article is part of the themed collections: Chemical Biology in OBC and New Talent

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Article information

DOI: https://doi.org/10.1039/C8OB02343C
Article type: Communication
Submitted: 20 Sept. 2018
Accepted: 19 Dec. 2018
First published: 21 Dec. 2018

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Org. Biomol. Chem., 2019,17, 1827-1833

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ortho-Fluoroazobenzene derivatives as DNA intercalators for photocontrol of DNA and nucleosome binding by visible light

B. Heinrich, K. Bouazoune, M. Wojcik, U. Bakowsky and O. Vázquez, Org. Biomol. Chem., 2019, 17, 1827 DOI: 10.1039/C8OB02343C

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Organic & Biomolecular Chemistry

ortho-Fluoroazobenzene derivatives as DNA intercalators for photocontrol of DNA and nucleosome binding by visible light†

Abstract

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ortho-Fluoroazobenzene derivatives as DNA intercalators for photocontrol of DNA and nucleosome binding by visible light

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