Ultrasensitive immunochromatographic strip for the detection of cyhalothrin in foods

Abstract

In this study, a highly specific and sensitive monoclonal antibody (mAb) against lambda-cyhalothrin (LCT) was prepared. The half maximal inhibitory concentration (IC₅₀) in the ic-ELISA was 1.2 ng mL⁻¹ and the limit of detection (LOD) value was 0.2 ng mL⁻¹. Based on the mAb, an immunochromatographic strip (ICS) was developed to qualitatively and quantitatively detect LCT in cabbage, parsley, spinach, and green tea samples. The results from the qualitative test can be observed with the naked eye within 10 min, and from quantitative detection experiments the linear detection ranges for cabbage, parsley, spinach, and green tea samples were 2.3–122.0, 1.5–98.2, 2.1–145.5, and 6.6–129.7 ng g⁻¹, respectively. Furthermore, recovery experiments were carried out at three spiked concentrations of LCT (5, 20, and 80 ng g⁻¹), and the recoveries of the ICS in vegetable samples ranged from 81.2% to 96.7%, with a coefficient of variation (CV) of less than 8.1%. For green tea, the recoveries of the ICS were from 80.4% to 90.7%, with a CV of less than 8.7%. The ICS assay established in this study can be used for the qualitative and quantitative determination of LCT residues in foods and agricultural products.