We reported a one-pot fluorescence-based assay to quantitively detect A3A activity combined with cytosine deamination and uracil excision. After deamination by A3A and USER enzyme treatment, the fluorescent turn-on effect at 520 nm was observed, which can be used to evaluate the A3A activity and screen inhibitors.
B. Wang, X. Zhang, Y. Wang, K. Chen, F. Wang, X. Weng and X. Zhou, RSC Chem. Biol., 2021, 2, 1201 DOI: 10.1039/D1CB00076D
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