Micro flow injection analysis of leucomalachite green in fish muscle using modified henequen fibers as microfluidic channels

Abstract

In this work a simple and novel procedure for leucomalachite green determination based on micro flow injection analysis (μFIA) with amperometric detection is presented. The method involves the use of henequen modified fibers as microfluidic channels. The μFIA system proposed offers a simple, rapid, and low-cost alternative for the determination. Capillary and gravitational forces across the modified henequen fibers control the flow rate, eliminating the need for external pumps. This technique requires low reagent consumption and allows portability for in situ measurements. The flow system is described, and the operational variables were studied and optimized using a Taguchi parameters design to increase analytical sensitivity. Under optimal conditions a limit of detection of 1.16 μg kg⁻¹ was achieved with adequate repeatability and reproducibility (expressed as %RSD <5.0%, n = 3, n = 9 respectively) in all cases. The effect of interfering species and the accuracy of the method were also investigated. The proposed methodology was validated and applied to determine LMG in tilapia muscle samples.