Cationic AIEgen micelle-improved chemiluminescent H2O2 assay by integrating reactant approach and CRET

Abstract

The enhancement of chemiluminescence (CL) intensity is significant in the development of chemiluminescent detection systems with improved sensitivity. In this study, a cationic surfactant with an intrinsic aggregation-induced emission emitter (AIEgen) has been applied to boost the CL signal of the horseradish peroxidase-luminol-H₂O₂ system. The formed cationic AIEgen micelles enhance the CL signal in two ways: the electrostatic attraction-mediated enrichment and approach of reactants and the high CRET efficiency between excited luminol radicals and AIEgen in the surfactant backbone. As a result, strong CL intensity is produced. Rapid and sensitive H₂O₂ detection is realized through the proposed cationic AIEgen micelle-containing chemiluminescent system with a limit of detection of 100 nM. The favourable selectivity over other possible interferents including metal ions and anions is due to the specific chemical reaction. Practical H₂O₂ analysis of thawing water samples with high accuracy using the proposed chemiluminescent platform is realized and is consistent with standard methods.