Self-assembly of DNA nanogels with endogenous microRNA toehold self-regulating switches for targeted gene regulation therapy

Jiaqi Yan; Haixia Zou; Wenhui Zhou; Xiaowan Yuan; Zhijun Li; Xiaodong Ma; Chang Liu; Yonghui Wang; Jessica M. Rosenholm; Wenguo Cui; Xiangmeng Qu; Hongbo Zhang

doi:10.1039/D2BM00640E

Self-assembly of DNA nanogels with endogenous microRNA toehold self-regulating switches for targeted gene regulation therapy†

Jiaqi Yan,‡^abc Haixia Zou,‡^a Wenhui Zhou,‡^c Xiaowan Yuan,^a Zhijun Li,^a Xiaodong Ma,^c Chang Liu,^c Yonghui Wang,^c Jessica M. Rosenholm,

^c Wenguo Cui,

^b Xiangmeng Qu

*^a and Hongbo Zhang

*^bc

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* Corresponding authors

^a Key Laboratory of Sensing Technology and Biomedical Instruments of Guangdong Province, School of Biomedical Engineering, Sun Yat-Sen University, 66 Gongchang Road, Shenzhen 518107, China
E-mail: quxm5@mail.sysu.cn

^b Department of Radiology, Shanghai Institute of Traumatology and Orthopaedics, RuiJin Hospital, Shanghai Jiao Tong University, 149 ChongqingNan Road, Shanghai, PR China
E-mail: hongbo.zhang@abo.fi

^c Pharmaceutical Sciences Laboratory and Turku Bioscience Center, Åbo Akademi University, FI-20520 Turku, Finland

Abstract

Herein, a smart nanohydrogel with endogenous microRNA-21 toehold is developed to encapsulate gemcitabine-loaded mesoporous silica nanoparticles for targeted pancreatic cancer therapy. This toehold mediated strand displacement method can simultaneously achieve specific drug release and miRNA-21 silencing, resulting in the up-regulation of the expression of tumor suppressor genes PTEN and PDCD4.

This article is part of the themed collection: microRNA and its role in gene regulation: Celebrating the 2024 Physiology or Medicine Nobel Prize

Biomaterials Science

Self-assembly of DNA nanogels with endogenous microRNA toehold self-regulating switches for targeted gene regulation therapy†

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Self-assembly of DNA nanogels with endogenous microRNA toehold self-regulating switches for targeted gene regulation therapy

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