A hydrogen-driven biocatalytic approach to recycling synthetic analogues of NAD(P)H

Holly A. Reeve; Jake Nicholson; Farieha Altaf; Thomas H. Lonsdale; Janina Preissler; Lars Lauterbach; Oliver Lenz; Silke Leimkühler; Frank Hollmann; Caroline E. Paul; Kylie A. Vincent

doi:10.1039/D2CC02411J

A hydrogen-driven biocatalytic approach to recycling synthetic analogues of NAD(P)H†

Holly A. Reeve,

‡*^a Jake Nicholson,

^a Farieha Altaf,^a Thomas H. Lonsdale,^a Janina Preissler,^b Lars Lauterbach,

^bc Oliver Lenz,

^b Silke Leimkühler,

^d Frank Hollmann,

^e Caroline E. Paul

*^e and Kylie A. Vincent

*^a

Author affiliations

* Corresponding authors

^a Department of Chemistry, University of Oxford, Inorganic Chemistry Laboratory, South Parks Road, Oxford, UK
E-mail: holly@hydregenoxford.com, kylie.vincent@chem.ox.ac.uk

^b Department of Chemistry, Technische Universität Berlin, Strasse des 17. Juni 135, 10623 Berlin, Germany

^c RWTH Aachen University iAMB – Institute of Applied Microbiology Worringer Weg 1, 52074 Aachen, Germany

^d Department of Molecular Enzymology, Institute of Biochemistry and Biology, University of Potsdam, Germany

^e Department of Biotechnology, Delft University of Technology, Van der Maasweg 9, 2629HZ Delft, The Netherlands
E-mail: c.e.paul@tudelft.nl

Abstract

We demonstrate a recycling system for synthetic nicotinamide cofactor analogues using a soluble hydrogenase with turnover number of >1000 for reduction of the cofactor analogues by H₂. Coupling this system to an ene reductase, we show quantitative conversion of N-ethylmaleimide to N-ethylsuccinimide. The biocatalyst system retained >50% activity after 7 h.

This article is part of the themed collections: Biocatalysis: A cross-journal collection and Celebrating 10 years of ChemComm Emerging Investigators

Chemical Communications

A hydrogen-driven biocatalytic approach to recycling synthetic analogues of NAD(P)H†

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A hydrogen-driven biocatalytic approach to recycling synthetic analogues of NAD(P)H

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