Reactive oxygen species on indoor surfaces

Abstract

Reactive oxygen species (ROS) are relatively unstable oxygen-containing radicals or non-radicals, some of which may react with tissues and biomolecules after entering the body. ROS is present in indoor aerosols, but it is unclear how much of that ROS is of indoor origin. Indoor surface films have been hypothesized to be a major source of the ROS observed on indoor aerosols. In this study, the ROS concentration on residential indoor surfaces was measured using a xylenol orange ferrous oxidation assay after wiping and extraction. On genuine surfaces frequently touched by apartment occupants, the concentration was >0.2 nmol cm⁻²; infrequently touched surfaces were at or below detection limits. On clean glass plates that had been deployed in apartments for 6 weeks, horizontal plates had higher concentrations than vertically oriented plates. The highest concentration, 1.3 nmol cm⁻², was observed on a horizontally oriented plate close to an electric stove. To simulate the dynamic oxidation of unsaturated hydrocarbons on indoor surfaces, a surface lipid mixture (SLM) was dosed on 19 glass plates which were then exposed to untreated laboratory air for periods ranging from 1 to 56 days. During the first 5–6 days, the ROS concentration increased roughly linearly to a maximum of 5–6 nmol cm⁻². Then the concentration ceased to increase, perhaps because reactive sites had become depleted. After 2 weeks, ROS decreased slowly, possibly due to a combination of volatilization, decomposition and continued formation by autoxidation. These field and laboratory results support the hypothesis that indoor surfaces can be a source of ROS.