Issue 4, 2003
From the journal:

Lab on a Chip

Sub-second isoelectric focusing in free flow using a microfluidic device

Yi Xu,†a   Chao-Xuan Zhang,‡a   Dirk Janasek§a  and  Andreas Manz*a  

* Corresponding authors

a Imperial College London, Chemistry Department, Exhibition Road, London, UK
E-mail: a.manz@imperial.ac.uk

Abstract

Using a microfabricated chip with a bed volume of 0.2 µL we demonstrate the validity of the scaling laws for molecular mass transport of isoelectric focusing (IEF) in free flow. Nano- or microlitre sample volumes can be concentrated within 430 ms by a factor of up to 400. These very fast performances make the chip applicable to proteomic analysis and for continuous monitoring of biochemical processes.

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Article information

DOI
https://doi.org/10.1039/B308476K
Article type
Communication
Submitted
22 Jūl. 2003
Accepted
17 Sept. 2003
First published
07 Okt. 2003

Lab Chip, 2003,3, 224-227

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Sub-second isoelectric focusing in free flow using a microfluidic device

Y. Xu, C. Zhang, D. Janasek and A. Manz, Lab Chip, 2003, 3, 224 DOI: 10.1039/B308476K

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