Issue 30, 2010
From the journal:

Chemical Communications

Transaminations with isopropyl amine: equilibrium displacement with yeast alcohol dehydrogenase coupled to in situcofactorregeneration†‡

Karim Engelmark Cassimjee,a   Cecilia Branneby,b   Vahak Abedi,c   Andrew Wellsd  and  Per Berglund*a  

* Corresponding authors

a Division of Biochemistry, School of Biotechnology, Royal Institute of Technology (KTH), AlbaNova University Centre, SE-106 91 Stockholm, Sweden
E-mail: per.berglund@biotech.kth.se
Fax: +46 8 5537 8468
Tel: +46 8 5537 8366

b Cambrex Karlskoga AB, SE-691 85 Karlskoga, Sweden
E-mail: cecilia.branneby@cambrex.com
Fax: +46 586 783129
Tel: +46 586 783000

c AstraZeneca R&D, SE-151 85 Södertälje, Sweden
E-mail: vahak.abedi@astrazeneca.com
Fax: +46 8 5532 4276
Tel: +46 8 5532 1766

d AstraZeneca Global Process R&D, Bakewell Road, Loughborough, Leics, UK
E-mail: andrew.wells@astrazeneca.com
Fax: +44 (0)1509 645588
Tel: +44 (0)1509 644439

Abstract

Enantiopure chiral amines synthesis using ω-transaminases is hindered by an unfavourable equilibrium, but when using isopropylamine as the amine donor the equilibrium can be completely displaced by using a specific dehydrogenase in situ for removal of formed acetone.

Graphical abstract: Transaminations with isopropyl amine: equilibrium displacement with yeast alcohol dehydrogenase coupled to in situ cofactor regeneration

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Article information

DOI
https://doi.org/10.1039/C0CC00050G
Article type
Communication
Submitted
21 Febr. 2010
Accepted
28 Apr. 2010
First published
12 Maijs 2010

Chem. Commun., 2010,46, 5569-5571

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Transaminations with isopropyl amine: equilibrium displacement with yeast alcohol dehydrogenase coupled to in situ cofactor regeneration

K. E. Cassimjee, C. Branneby, V. Abedi, A. Wells and P. Berglund, Chem. Commun., 2010, 46, 5569 DOI: 10.1039/C0CC00050G

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