Turn-On Fluorescent Probe Based on Dicyanisophorone for Rapid Detecting of Peroxynitrite in Aqueous Medium and Bioimaging

Abstract

One kind of novel dicyanisophorone-based colorimetric fluorescent probe 3 has been prepared for recognizing peroxynitrite in aqueous condition. Large bathochromic shift of the absorption band is observed upon titration with ONOO–, inducing a clear solution color change from yellow to pale pink which enabling “naked-eye” detection possible. Moreover, probe 3 can react instantly with ONOO–accompanied by significant fluorescence enhancement at 621 nm while the detection limit is as low as 37 nM. Most importantly, probe 3 exhibits high selectivity and sensitivity towards ONOO– over other competitive ions in the aqueous solutions. Probe 3 has also been successfully applied in living MCF-7 cells and the result suggests that probe 3, could be applied as a potential candidate for the detection of ONOO–.