Themed collection Analyst HOT Articles 2024

This review discusses the formation and manipulation of magnetic droplets on microfluidic chips and classifies related detection techniques. Applications in biomedicine were reviewed. Future development of this emerging field is provided.

Raman microscopy can reveal metabolic signatures and heterogeneity at the single cell level.

A myrid of aptamers were isolated thus far against SARS-CoV-2 by using in silico selection.

This review summarizes sensitive analytical detection. Analyte pre-concentration by droplet deposition is combined with SERS enhancement.

This minireview summarizes the recent advancements in impact electrochemistry-based biosensing over the past two to three years and provides insights into the future directions of this field.

This review aims to introduce the development of DNA nanotechnology and recent advances of DNA nanostructure-based delivery systems for bioimaging, disease diagnosis, and therapeutic applications.

Surface-enhanced Raman scattering (SERS) is a very important tool in vibrational spectroscopy.

The research progress of MOF-based nanozymes with oxidoreductase-like activity.

The abnormal expression of human DNA methyltransferases (DNMTs) is closely related with the occurrence and development of a wide range of human cancers.

Photoactivated fluorophores (PAFs) are highly effective imaging tools that exhibit a removal of caging groups upon light excitation, resulting in the restoration of their bright fluorescence.

The recent advances of MOF-based SERS substrates in quantitative analysis of food contaminants are reviewed including properties, main roles, categories, structures, preparation methods, and food-safety applications.

Neurobiological research relies heavily on imaging techniques, such as fluorescence microscopy, to understand neurological function and disease processes.

A comprehensive review on the properties and versatile applications of fluorescent carbon dots in detecting nucleic acids, imaging nucleoli, and delivering genes.

Patients with end-stage kidney disease (ESKD) rely on dialysis to remove toxins and stay alive.

Overview of recent studies on SERS for biomolecular complexities analysis.

Different purification techniques for carbon dots.

Overview of nanopores for single cell detection.

This review summarizes the recent advances in three-dimensional graphene structures, including preparation methods and their detection application in the biomedical field.

Synthesis, characterization, and application of carbon dots@noble metal nanocomposites.

Fluorometric, colorimetry, chemiluminescence, SERS, and dual-mode techniques for biosensing and imaging of telomerase are summerized. Relevant signal amplification strategies are included to provide ideas for exploration of more practicable methods.

Herein the development of cellular impedance biosensors, electrochemical impedance spectroscopy, and the general principles and terms associated with the cell–electrode interface is reviewed.

Seeing is believing - and optical sensors (optodes) are ideal tools to make chemistry visible in real time. Find out how technological and chemical advances enable multiparameter imaging of complex samples.

The photovoltage (V_oc) of n-Si/Au photoelectrodes varies with the solution potential (E⁰) when E⁰ falls between the valence and conduction band edges of the semiconductor but is constant when E⁰ is more positive than the valence band edge.

Visual electrochemiluminescence (ECL) emission from L012 and hydrogen peroxide is generated from an all-solid-state electrochemical cell with a polyacrylamide hydrogel as the solid electrolyte.

A novel green and eco-friendly carbon-based nanoprobe was developed for dual-channel analysis of tartrazine (Trz) and Pd(II) involving, respectively, fluorescence resonance energy transfer (FRET) and electron transfer mechanisms.

We report here a tunable proton platform for the tumor micro-acid environment that is used for tumor imaging and treatment.

Pure shift NMR experiments greatly enhance spectral resolution by collapsing multiplet structures into singlets and, with optimal water suppression, can be used for aqueous samples.

Enzymes (1) and nanozymes (2) have significant differences in the Michaelis–Menten constant. Detection of nanozymes using enzyme substrates results in lower catalytic activity and a significantly higher limit of detection.

Graphene oxide can increase the levels of intracellular H₂O₂ and ˙OH, and cause the irreversible oxidative modification. This leads to the activation of the JNK/p38 signaling pathway and promote hepatocyte apoptosis.

A split G-quadruplex based PfAgo sensing platform was developed for nucleotide mutation discrimination and human genotyping.

The development of an in situ combination of AFM and soft X-ray μXRF in a single instrument, with AFM integration accomplished without substantial original set-up alteration.

An electrochemical sensing approach for ultrasensitive MTase activity assay is proposed based on cascade strand displacement polymerization after specific cleavage reaction in the methylated state.

Dipicolinic acid (DPA) is a key biomarker of bacterial spores.

A NIR FUCL probe NIRX-1 was proposed and applied to rapid and sensitive visualization of HSO₃⁻in vitro and in vivo.

A reliable ratiometric fluorescence probe (ZIF-9-TCPP) based on FRET was designed for Zn²⁺ detection.

A TIRF microscopy-based method for single vesicle aggregation analysis enables precise assessment of EV aggregation under various conditions, enhancing EV characterization and functional analysis.

For living HeLa cells, we report a small and minimally-invasive Raman reporter (about 2 aa and <1 kDa), which can be site-specifically introduced into proteins by genetic codon expansion combined with tetrazine ligation.

Using capture-SELEX, short aptamers for metronidazole and ibuprofen were isolated and they can be used for the detection of these drugs illicitly added to soft drinks.

Acute lymphoblastic leukaemia (ALL) is a complex disease in pediatric oncology, necessitating accurate diagnostic strategies for effective treatment planning.

An automated microscope image detection device (A-MMD) is designed to detect fluorescent droplets in droplet digital PCR images captured by multiple microscopes.

Utilizing the specific interaction between carboxylic groups and ammonia gas has led to the development of an ultra-sensitive ammonia sensor based on a quartz crystal microbalance using nanofibers overlaid with carboxylic group-functionalized MWCNTs.

A highly efficient photoelectrochemical (PEC) strategy was proposed for the determination of ascorbic acid (AA).

The difference in kinetic information among PtRu bimetallic nanocatalysts, Pt and Ru monometallic nanocatalysts is revealed by the single-molecule fluorescence technique.

Surface-enhanced Raman spectroscopy (SERS) and partial least squares regression was utilized to determine the dominant bacteria in mixtures of two common vaginal microbes in both pure and complex fluids.

Multi-analytical approach to reveal the presence and distribution of lead compounds at the micro-scale level in the red stains on heritage marbles.

Saliva samples are analysed by using infrared (IR) spectroscopy with computational tools to diagnose lung cancer patients in a screening program study.

Strategies based on nanomaterials for sterilization address the problem of antibiotic resistance faced by conventional antimicrobials, with the contribution of photocatalytic compounds being particularly prominent.

Nanozymes offer several benefits including cost-effectiveness, enzyme-like catalytic abilities, enhanced stability, adjustable catalytic activity, easy recyclability, mild reaction conditions, and environmental friendliness.

4-methylene-N-alkyl dihydropyridine derivatives bind preferentially to the FA 1 site and are used to quantitatively estimate HSA levels in blood and serum.

We studied the impact of zwitterionic surfactants on three different sensing mechanisms, nanomaterial encapsulation, and compatibility with different fabrication approaches.

The “antenna effect” is one of the most important energy transfer modes in lanthanide light-emitting polymers.

THz reveals the interaction between L-Glu and the NMDAR involving water molecules.

A novel near-infrared fluorescent probe was developed for selectively monitoring hydrogen sulfide (H₂S). This probe was successfully applied to detect H₂S in living cells, zebrafish, and hippocampal tissue of Alzheimer's disease (AD) mouse models.

Ultrasensitive detection of the H5N1 nucleic acid fragment by ICP-MS using DNA dendrimer-carried AgNP labeling was developed. This approach enabled significant signal amplification, while magnetic separation effectively suppressed background signals.

The corrective protocol predicts sensor response from paper-based solution sampling potentiometric measurement, offering a simple method to correct the readout. This eliminates the need to modify the paper substrate for measuring heavy metal ions.

Robust machine learning scheme using analytical data to improve photoanode performance.

Latent fingermarks on non-conducting surfaces such as glass, ceramics, and plastics can be visualized electrochromically using benzyl viologen and with the assistance of gold layer.

A non-invasive, label-free and high-precision diagnosis of Helicobacter pylori (H. pylori) infection is conducted by using a dual-element multimodal gas sensor array.

New fluorogenic indicators for HIV-1 TAR RNA have been developed for fluorescence indicator displacement (FID) assay based on forced intercalation of thiazole orange unit integrated into the middle of transactivator of transcription (Tat) peptide.

A facile, green method and scalable synthesis of MnO₂ nanosheets by mixing Shiranui mandarin orange juice and KMnO₄. A rapid and robust colorimetric assay was developed for the quantification of GSH and cTnI based on MnO₂ nanosheets-TMB system.

Our dual-mode FI and FP aptamer probe detects P-glycoprotein in drug-resistant ovarian cancer cells, demonstrating high specificity and sensitivity with detection limits of 1.13 nM and 40 cells per mL, promising improved diagnosis strategies.

Schemetic diagram of preparation of MXene–peptide composites.

It is known that the abnormal expression of specific cellular miRNAs is closely related to cell apoptosis, and so monitoring the level change of these miRNAs can in principle be used to evaluate the process of apoptosis stimulated by drugs.

An electrochemical aptamer biosensor based on the CRISPR-Cas12a system was constructed for the detection of cancer tumor biomarker MUC1 mucin, the sensitivity was significantly improved by employing the enzyme catalytic reaction.

Combining target-initiated 3D DNAzyme motors with single-molecule imaging enables intuitive, sensitive and rapid detection of different miRNAs. Dual miRNA detection of EVs using this method is effective for clinical diagnosis of colorectal cancer.

An organ-on-a-chip in vitro human model of the blood–brain barrier (BBB) was applied to iteratively enrich for AAV variants that efficiently cross the BBB and transduce astrocyte cells.

We quantified the dissolution rate of sub-nL oil microdroplets in a bulk aqueous phase based on the evolution of cyclic voltammograms recorded for a redox mediation confined in the microdroplet.

A magneto-electrochemical method for enhanced chiral recognition of amino acid enantiomers has been developed.

A binding-triggered hybridization chain reaction cascade multi-site activated CRISPR/Cas12a signal amplification strategy for sensitive detection of α-synuclein. This work provides a promising analysis tool for the early diagnosis of Parkinson's disease.

A periodic mesoporous organosilica (PMO)-based heteromembrane with hierarchical pore architecture and glutathione (GSH)-responsive switchable wettability is synthesized for sensitive and efficient GSH detection.