Engineering of D-fructose-6-phosphate aldolase A for improved activity towards cinnamaldehyde

Xiaohong Yang; Lidan Ye; Aipeng Li; Chengcheng Yang; Huilei Yu; Jiali Gu; Fei Guo; Ling Jiang; Fan Wang; Hongwei Yu

doi:10.1039/C6CY01622G

Engineering of d-fructose-6-phosphate aldolase A for improved activity towards cinnamaldehyde†

Xiaohong Yang,^a Lidan Ye,^a Aipeng Li,^a Chengcheng Yang,^a Huilei Yu,^b Jiali Gu,^c Fei Guo,^a Ling Jiang,^d Fan Wang^a and Hongwei Yu

*^a

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* Corresponding authors

^a Institute of Bioengineering, College of Chemical and Biological Engineering, Zhejiang University, Hangzhou 310027, PR China
E-mail: yuhongwei@zju.edu.cn

^b State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, China

^c College of Life Science, Department of Materials Chemistry, Huzhou University, Huzhou 313000, PR China

^d Collaborative Innovation Center of Yangtze River Delta Green Pharmaceuticals, Zhejiang University of Technology, Hangzhou 310014, PR China

Abstract

D-Fructose-6-phosphate aldolase A (FSAA) from Escherichia coli was engineered for enhanced catalytic efficiency towards cinnamaldehyde. The result implies that the site 59 residue possibly affects the activity of FSAA towards cinnamaldehyde through a residue interaction network rather than binding to the catalytic water as generally regarded.

This article is part of the themed collection: 2017 Catalysis Science & Technology HOT Articles

Catalysis Science & Technology

Engineering of d-fructose-6-phosphate aldolase A for improved activity towards cinnamaldehyde†

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Engineering of D-fructose-6-phosphate aldolase A for improved activity towards cinnamaldehyde

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