Incorporation of chlorinated analogues of aliphatic amino acids during cell-free protein synthesis

Dannon J. Stigers; Zachary I. Watts; James E. Hennessy; Hye-Kyung Kim; Romeo Martini; Matthew C. Taylor; Kiyoshi Ozawa; Jeffrey W. Keillor; Nicholas E. Dixon; Christopher J. Easton

doi:10.1039/C0CC02879G

Incorporation of chlorinated analogues of aliphatic amino acids during cell-free protein synthesis†‡

Dannon J. Stigers,^a Zachary I. Watts,^a James E. Hennessy,^a Hye-Kyung Kim,^a Romeo Martini,^a Matthew C. Taylor,^b Kiyoshi Ozawa,^c Jeffrey W. Keillor,§^a Nicholas E. Dixon^c and Christopher J. Easton*^a

Author affiliations

* Corresponding authors

^a ARC Centre of Excellence for Free Radical Chemistry and Biotechnology, Research School of Chemistry, Australian National University, Canberra ACT 0200, Australia
E-mail: easton@rsc.anu.edu.au

^b CSIRO Entomology, Canberra ACT 2601, Australia

^c School of Chemistry, University of Wollongong, Wollongong NSW 2522, Australia

Abstract

3-Chloro-Abu and 4-chloro-Nva are biosynthetically incorporated into E. coli peptidyl-Pro cis-trans isomerase B, as substitutes for Val and Leu, respectively. The extent of incorporation is up to ∼90%, and substituted protein is catalytically active. By contrast, 4-chloro-Val is not an effective replacement for Ile.

This article is part of the themed collection: Enzymes and Proteins

Chemical Communications

Incorporation of chlorinated analogues of aliphatic amino acids during cell-free protein synthesis†‡

Abstract

Supplementary files

Article information

Download Citation

Permissions

Incorporation of chlorinated analogues of aliphatic amino acids during cell-free protein synthesis

Social activity

Search articles by author

Spotlight

Advertisements