Issue 3, 2011
From the journal:

Chemical Communications

Screening of a minimal enriched P450 BM3 mutant library for hydroxylation of cyclic and acyclic alkanes†

Evelyne Weber,ab   Alexander Seifert,a   Mihaela Antonovici,a   Christopher Geinitz,a   Jürgen Pleissa  and  Vlada B. Urlacher*b  

* Corresponding authors

a Institute of Technical Biochemistry, Universitaet of Stuttgart, Allmandring 31, 70569 Stuttgart, Germany

b Institute of Biochemistry, Heinrich-Heine University Düsseldorf, Universitätsstr. 1, 40225 Düsseldorf, Germany
E-mail: Vlada.Urlacher@uni-duesseldorf.de

Abstract

A minimal enriched P450 BM3 library was screened for the ability to oxidize inert cyclic and acyclic alkanes. The F87A/A328V mutant was found to effectively hydroxylate cyclooctane, cyclodecane and cyclododecane. F87V/A328F with high activity towards cyclooctane hydroxylated acyclic n-octane to 2-(R)-octanol (46% ee) with high regioselectivity (92%).

Graphical abstract: Screening of a minimal enriched P450 BM3 mutant library for hydroxylation of cyclic and acyclic alkanes

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Article information

DOI
https://doi.org/10.1039/C0CC02924F
Article type
Communication
Submitted
30 jul 2010
Accepted
27 okt 2010
First published
15 nov 2010

Chem. Commun., 2011,47, 944-946

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Screening of a minimal enriched P450 BM3 mutant library for hydroxylation of cyclic and acyclic alkanes

E. Weber, A. Seifert, M. Antonovici, C. Geinitz, J. Pleiss and V. B. Urlacher, Chem. Commun., 2011, 47, 944 DOI: 10.1039/C0CC02924F

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