Issue 31, 2017

Stacking modular DNA circuitry in cascading self-assembly of spherical nucleic acids

Abstract

Unwanted initial and asymptotic leakages are vital to fabricating integrated circuitries. This paper presents a novel strategy that combines simulations and experiments to extend the circuitries of spherical nucleic acid (SNA) to three layers. The results indicate that SNA-based circuits are insensitive to initial leakage but are significantly influenced by asymptotic leakage, particularly for the two-layer circuit. The dynamic behaviors of and the effects of leakage on the performance of the integrated system can be regulated by fine tuning of toehold domains and the molar ratio of the species. Small upstream layers and a large downstream layer are experimentally adopted to balance the stability and operation efficiency of the integrated three-layer circuitry. This work soundly demonstrates the capability of fabricating modular cascaded circuitry for driving SNA assembly in sophisticated systems, and modulating or programming the molecular machinery for developing an autonomous system.

Graphical abstract: Stacking modular DNA circuitry in cascading self-assembly of spherical nucleic acids

Supplementary files

Article information

Article type
Paper
Submitted
13 Mei 2017
Accepted
14 Jul 2017
First published
17 Jul 2017

J. Mater. Chem. B, 2017,5, 6256-6265

Stacking modular DNA circuitry in cascading self-assembly of spherical nucleic acids

D. Yao, S. Xiao, X. Zhou, H. Li, B. Wang, B. Wei and H. Liang, J. Mater. Chem. B, 2017, 5, 6256 DOI: 10.1039/C7TB01307H

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements