Issue 31, 2020

Tuning hydrogel properties with sequence-defined, non-natural peptoid crosslinkers

Abstract

The native extracellular matrix (ECM) is composed of hierarchically structured biopolymers containing precise monomer sequences and chain shapes to yield bioactivity. Recapitulating this structure in synthetic hydrogels is of particular interest for tissue engineering and in vitro disease models to accurately mimic biological microenvironments. However, despite extensive research on hydrogels, it remains a challenge to recapitulate the hierarchical structure of native ECM with completely synthetic hydrogel platforms. Toward this end, this work presents a synthetic hydrogel system using commercially available poly(ethylene glycol) macromers with sequence-defined poly(N-substituted glycines) (peptoids) as crosslinkers. We demonstrate that bulk hydrogel mechanics, specifically as shear storage modulus, can be controlled by altering peptoid sequence and structure. Notably, the helical peptoid sequence investigated here increases the storage modulus of the resulting hydrogels with increasing helical content and chain length, in a fashion similar to helical peptide-crosslinked hydrogels. In addition, the resulting hydrogels are shown to be hydrolytically and enzymatically stable due to the N-substituted peptidomimetic backbone of the crosslinkers. We further demonstrate the potential utility of these peptoid-crosslinked hydrogels as a viable cell culture platform using seeded human dermal fibroblasts in comparison to peptide-crosslinked hydrogels as a control. Taken together, our system offers a strategy toward ECM mimics that replicate the hierarchy of biological matrices with completely synthetic, sequence-defined molecules.

Graphical abstract: Tuning hydrogel properties with sequence-defined, non-natural peptoid crosslinkers

Supplementary files

Article information

Article type
Paper
Submitted
12 Mac 2020
Accepted
14 Mei 2020
First published
14 Mei 2020

J. Mater. Chem. B, 2020,8, 6925-6933

Author version available

Tuning hydrogel properties with sequence-defined, non-natural peptoid crosslinkers

L. D. Morton, A. Hillsley, M. J. Austin and A. M. Rosales, J. Mater. Chem. B, 2020, 8, 6925 DOI: 10.1039/D0TB00683A

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements