Issue 45, 2023

Molecular beacon decorated silver nanowires for quantitative miRNA detection by a SERS approach

Abstract

Advantages of biosensors based on surface enhanced Raman scattering (SERS) rely on improved sensitivity and specificity, and suited reproducibility in detecting a target molecule that is localized in close proximity to a SERS-active surface. Herein, a comprehensive study on the realization of a SERS biosensor designed for detecting miRNA-183, a miRNA biomarker that is specific for chronic obstructive pulmonary disease (COPD), is presented. The used strategy exploits a signal-off mechanism by means of a labelled molecular beacon (MB) as the oligonucleotide biorecognition element immobilized on a 2D SERS substrate, based on spot-on silver nanowires (AgNWs) and a multi-well low volume cell. The MB was properly designed by following a dedicated protocol to recognize the chosen miRNA. A limit of detection down to femtomolar concentration (3 × 10−16 M) was achieved and the specificity of the biosensor was proved. Furthermore, the possibility to regenerate the sensing system through a simple procedure is shown: with regeneration by using HCl 1 mM, two detection cycles were performed with a good recovery of the initial MB signal (83%) and a reproducible signal after hybridization.

Graphical abstract: Molecular beacon decorated silver nanowires for quantitative miRNA detection by a SERS approach

Supplementary files

Article information

Article type
Paper
Submitted
19 Sep 2023
Accepted
26 Okt 2023
First published
27 Okt 2023
This article is Open Access
Creative Commons BY license

Anal. Methods, 2023,15, 6165-6176

Molecular beacon decorated silver nanowires for quantitative miRNA detection by a SERS approach

M. Banchelli, S. Tombelli, M. de Angelis, C. D'Andrea, C. Trono, F. Baldini, A. Giannetti and P. Matteini, Anal. Methods, 2023, 15, 6165 DOI: 10.1039/D3AY01661G

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