Issue 9, 2023

Development of substrates for the culture of human pluripotent stem cells

Abstract

Although human pluripotent stem cell (hPSC) lines were initially established in culture using feeder cells, the development of culture media and substrates is essential for safe, stable, high-quality, and efficient production of large numbers of cells. Many researchers are now culturing hPSCs in chemically defined media and on culture substrates without feeder cells. In this review, we first discuss the problems with Matrigel, which has long been used as a culture substrate. Then, we summarize the development of extracellular matrix proteins for hPSCs, which are now the mainstream alternative, and synthetic substrates that are expected to be the future mainstream alternative. We also highlight three-dimensional culture for suitable mass production of hPSCs.

Graphical abstract: Development of substrates for the culture of human pluripotent stem cells

Article information

Article type
Minireview
Submitted
11 Sep 2022
Accepted
22 Feb 2023
First published
15 Mac 2023
This article is Open Access
Creative Commons BY-NC license

Biomater. Sci., 2023,11, 2974-2987

Development of substrates for the culture of human pluripotent stem cells

E. Kawase and N. Nakatsuji, Biomater. Sci., 2023, 11, 2974 DOI: 10.1039/D2BM01473D

This article is licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported Licence. You can use material from this article in other publications, without requesting further permission from the RSC, provided that the correct acknowledgement is given and it is not used for commercial purposes.

To request permission to reproduce material from this article in a commercial publication, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party commercial publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements