Dali Sun, Li Yang, Christopher J. Lyon and Tony Hu
J. Mater. Chem. B, 2020,8, 5416-5419
DOI:
10.1039/D0TB00350F,
Communication
Nanoparticle-enhanced assays read by high-magnification dark-field microscopy require time-intensive analysis methods subject to selection bias, which can be resolved by using low magnification dark-field assays (LMDFA), at the cost of reduced sensitivity. We have simulated and experimentally validated a tunable linker-based signal amplification strategy yielding 6-fold enhanced LMDFA sensitivity.