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Abstract | Cited by

Nanoparticle-enhanced assays read by high-magnification dark-field microscopy require time-intensive analysis methods subject to selection bias, which can be resolved by using low magnification dark-field assays (LMDFA), at the cost of reduced sensitivity. We have simulated and experimentally validated a tunable linker-based signal amplification strategy yielding 6-fold enhanced LMDFA sensitivity.

Graphical abstract: Simulation-directed amplifiable nanoparticle enhanced quantitative scattering assay under low magnification dark field microscopy

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