Combined detection of hepatitis B virus surface antigen and hepatitis B virus DNA using a DNA sensor

Abstract

Hepatitis B virus (HBV) infection is associated with the progression of liver disease. Occult HBV infection (OBI) is defined as the existence of detectable HBV DNA in HBV surface antigen negative individuals. However, HBV DNA is negative in serum while HBV surface antigen remains positive in incompletely cured chronic HBV infection. Hence, combined detection of HBV surface antigen and HBV DNA is essential for the accurate detection and rehabilitation of HBV infection. Therefore, a multiplex detection strategy based on branched DNA nanostructures was developed. The single-stranded segment of a branched DNA nanostructure (segments of S4 and S2) assembled by four single-stranded DNA was hybridized with the aptamer of HBV surface antigen and DNA hairpin to construct a DNA nanosensor, which can achieve high specificity identification and highly sensitive fluorescence responses to the targets. The detection limits of the developed nanosensor for HBV and HBV DNA are 50 pM and 5 nM, respectively. The combined detection of HBV surface antigen and HBV DNA provides a new insight for more thorough diagnostic evaluation of HBV infection and rehabilitation.

Graphical abstract: Combined detection of hepatitis B virus surface antigen and hepatitis B virus DNA using a DNA sensor

Supplementary files

Article information

Article type
Paper
Submitted
03 Sep 2024
Accepted
23 Sep 2024
First published
24 Sep 2024

Anal. Methods, 2024, Advance Article

Combined detection of hepatitis B virus surface antigen and hepatitis B virus DNA using a DNA sensor

H. Gong, S. Yao, Y. Li, C. Chen, F. Chen and C. Cai, Anal. Methods, 2024, Advance Article , DOI: 10.1039/D4AY01629G

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