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Abstract | Cited by

The development of biocompatible two-photon fluorophores with a large absorption cross-section is challenging, despite the presence of theoretical guidelines. By rendering asymmetric PRODAN dye centrosymmetric, we designed and synthesized a novel class of two-photon fluorophores (TPZ). Their photophysical properties were investigated and their imaging potentials in cells, tissues and zebrafish were showcased.

Graphical abstract: TPZ, a bright centrosymmetric two-photon scaffold for bioimaging

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